Processes For Preparing AG-10, Its Intermediates, and Salts Thereof

ABSTRACT

as well as crystalline types of Formula IX (AG-10). The processes described herein provide improved yields and efficiency, while the pharmaceutically acceptable salts and crystalline forms provide unexpected pharmacokinetic properties. Other features and aspects of the present disclosure will be apparent to a person of skill in the art upon reading the remainder of the specification.

CROSS-REFERENCES TO RELATED APPLICATIONS

This application claims the benefit of priority under 35 U.S.C § 119(e) to U.S. Provisional Application Ser. No. 62/460,576 filed Feb. 17, 2017, the disclosure of which is incorporated herein by reference in its entirety.

STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT

NOT APPLICABLE

REFERENCE TO A “SEQUENCE LISTING,” A TABLE, OR A COMPUTER PROGRAM LISTING APPENDIX SUBMITTED ON A COMPACT DISK

NOT APPLICABLE

BACKGROUND OF THE INVENTION

Aberrant protein interaction and aggregation, either through protein misfolding or over activation of a signaling pathway is the underlying cause of a large number of human degenerative diseases. As such, targeting protein protein interactions (PPIs) is of therapeutic interest.

To date approved inhibitors of PPIs are proteins rather than small-molecules inhibitors. For example, therapeutic monoclonal antibodies (mAbs) are use in treating cancer, autoimmune, infectious, and neuodegenerative diseases. Therapeutic mAbs are costly to manufacture, they require administration by injection, and can illicit an immune-response in the patient. For these reasons the development of small-molecule inhibitors of PPIs is of interst.

One such example of aberrant protein aggregation is the soluble protein transthyretin (TTR or prealbumin). TTR is a 55 kDa homotetrameric protein present in blood and cerebrospinal fluid. When dissociated from its homoterameric form, TTR dimers can misfold into amyloidogenic monomers. This has been observed with the wild type TTR as well as more than 100 different mutated variants. Research has shown that stabilizing the tetrameric form of TTR inhibits the misfolding of amyloidogenic monomers and subsequent TTR amyloid formation.

Recent work has identified 3-(3-(3,5-dimethyl-1H-pyrazol-4-yl)propoxy)-4-fluorobenzoic acid (AG-10) as a promising candidate to treat TTR amyloid related diseases such as TTR amyloid cardiomyopathy. This compound has been disclosed in WO 2014/100227. Notably, the disclosure does not provide any additional forms of AG-10 and the method of synthesis described would not be suitable for industrial manufacturing.

As such, there exists a need to produce improved methods of synthesizing AG-10 and to provide additional forms of AG-10 that offer advantageous pharmacokinetic properties. The present disclosure addresses these needs and provides related advantages as well.

BRIEF SUMMARY

In one aspect, the present disclosure provides an improved method for prepairing a compound of Formula IX

comprising

-   -   (a) contacting a compound of Formula II

-   -   with a compound of Formula III

-   -   a first base, and a first organic solvent to provide a compound         of Formula IV

-   -   wherein each R¹ is independently a halogen or a sulfonate ester;     -   (b) contacting a compound of Formula IV with hydrazine and a         second organic solvent to provide a compound of Formula V

-   -   (c) contacting a compound of Formula V with a sulfonating agent         or halogenating agent to provide a compound of Formula VI

-   -   wherein R² is a halogen or a sulfonate ester;     -   (d) contacting a compound of Formula VI with a compound of         Formula VII

-   -   a second base, and an third organic solvent to provide a         compound of Formula VIII

-   -   wherein R³ is selected from the group consisting of an C₁₋₁₂         alkyl, C₂₋₁₂ alkenyl, C₁₋₁₂ alkynyl, C₃₋₈cycloalkyl,         heterocycloalkyl, aryl, and heteroaryl, each of which is         optionally substituted.     -   (e) contacting a compound of Formula VIII with a third base to         provide a compound of Formula IX.

In a second aspect, the present disclosure provides a phamraceutically acceptable salt respresented by Formula I or Ib

wherein X is a pharmaceutically acceptable anion of a protic acid, and Y is a multiprotic acid.

In a third aspect, the present disclosure provides crystalline types A-K of Formula IX.

Other features, elements, and aspects of the present disclosure will be apparent from the accompanying drawings and from the detailed description that follows.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 provides a scheme as described herein for the preparation of AG-10 and its intermediates.

FIG. 2 shows pharmacokinetic results demonstrating the high bioavailability of AG-10 in multiple species.

FIG. 3 shows pharmacokinetic results demonstrating the high bioavailability of AG-10 in male and female dogs at different dosing levels.

FIG. 4 shows an X-ray powder diffraction (XRPD) pattern of the mesylate salt of Formula IX.

FIG. 5 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of the mesylate salt of Formula IX.

FIG. 6 shows an X-ray powder diffraction (XRPD) pattern of the edisylate salt of Formula IX.

FIG. 7 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of the edisylate salt of Formula IX.

FIG. 8 shows an X-ray powder diffraction (XRPD) pattern of the besylate salt of Formula IX.

FIG. 9 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of the besylate salt of Formula IX.

FIG. 10 shows an X-ray powder diffraction (XRPD) pattern of the tosylate salt of Formula IX.

FIG. 11 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of the tosylate salt of Formula IX.

FIG. 12 shows an X-ray powder diffraction (XRPD) pattern of the esylate salt of Formula IX.

FIG. 13 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of the esylate salt of Formula IX.

FIG. 14 shows an X-ray powder diffraction (XRPD) pattern of the bromide salt of Formula IX.

FIG. 15 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of the bromide salt of Formula IX

FIG. 16 shows an X-ray powder diffraction (XRPD) pattern of form a of the nitrate salt of Formula IX.

FIG. 17 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of form a of the nitrate salt of Formula IX.

FIG. 18 shows an X-ray powder diffraction (XRPD) pattern of form b of the nitrate salt of Formula IX.

FIG. 19 shows an X-ray powder diffraction (XRPD) pattern of the sulfate salt of Formula IX.

FIG. 20 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of the sulfate salt of Formula IX

FIG. 21 shows an X-ray powder diffraction (XRPD) pattern of the oxalate salt of Formula IX.

FIG. 22 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of the oxalate salt of Formula IX.

FIG. 23 shows an X-ray powder diffraction (XRPD) pattern of form a of the maleate salt of Formula IX.

FIG. 24 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots form a of the maleate salt of Formula IX.

FIG. 25 shows an X-ray powder diffraction (XRPD) pattern of form b of the maleate salt of Formula IX.

FIG. 26 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of form b of the maleate salt of Formula IX.

FIG. 27 shows an X-ray powder diffraction (XRPD) pattern of the acetic acid salt of Formula IX.

FIG. 28 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of the acetic acid salt of Formula IX.

FIG. 29 shows an X-ray powder diffraction (XRPD) pattern of the L-malic acid salt of

Formula IX.

FIG. 30 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of the L-malic acid salt of Formula IX.

FIG. 31 shows an X-ray powder diffraction (XRPD) pattern of crystalline Type A of Formula IX (3 different samples).

FIG. 32, FIG. 33, FIG. 34 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of crystalline Type A of Formula IX.

FIG. 35 shows a polarized light microscopy (PLM) image of crystalline Type A of Formula IX.

FIG. 36 shows the asymmetric unit of crystalline Type A of Formula IX.

FIG. 37 shows dynamic vapor sorption (DVS) data of crystalline Type A of Formula IX.

FIG. 38 shows X-ray powder diffraction (XRPD) patterns of crystalline Type A of Formula IX before (lower) and after (upper) DVS.

FIG. 39 shows a diagram summarizing the interconversions between identified crystal forms Type A, B, C, E, G, H, I, J.

FIG. 40 shows an X-ray powder diffraction (XRPD) pattern of crystalline Type B of Formula IX.

FIG. 41 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of crystalline Type B of Formula IX.

FIG. 42 shows X-ray powder diffraction (XRPD) patterns of crystalline Type B of Formula IX before heating (top), heating to 100° C. (second from top), heating to 170° C. (second from bottom), and crystalline Type I reference (bottom). Upon heating, Type B converts to Type I.

FIG. 43 shows an X-ray powder diffraction (XRPD) pattern of crystalline Type C of Formula IX.

FIG. 44 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of crystalline Type C of Formula IX.

FIG. 45 shows an X-ray powder diffraction (XRPD) pattern of crystalline Type D of Formula IX (upper plot) and crystalline Type F of Formula IX (lower plot).

FIG. 46 shows an X-ray powder diffraction (XRPD) pattern of crystalline Type E of Formula IX.

FIG. 47 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of crystalline Type E of Formula IX.

FIG. 48 shows X-ray powder diffraction (XRPD) patterns of crystalline Type E of

Formula IX before heating (top), heating to 195° C. (middle), and crystalline Type I reference (bottom). Upon heating, Type E converts to Type I.

FIG. 49 shows an X-ray powder diffraction (XRPD) pattern of crystalline Type G of Formula IX.

FIG. 50 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of crystalline Type G of Formula IX.

FIG. 51 shows an X-ray powder diffraction (XRPD) pattern of crystalline Type H of Formula IX.

FIG. 52 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of crystalline Type H of Formula IX.

FIG. 53 shows ¹H NMR spectrum of crystalline Type H of Formula IX.

FIG. 54 shows X-ray powder diffraction (XRPD) patterns of crystalline Type H of

Formula IX before heating (top), heating to 120° C. (middle), and crystalline Type I reference (bottom). Upon heating, Type H converts to Type I.

FIG. 55 shows an X-ray powder diffraction (XRPD) pattern of crystalline Type I of Formula IX.

FIG. 56 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of crystalline Type I of Formula IX.

FIG. 57 shows X-ray powder diffraction (XRPD) patterns of crystalline Type I of Formula IX (reference, top), before N₂ purging (second from top), after N₂ purging for 1.5 hrs (second from bottom), and crystalline Type B reference (bottom). Upon N₂ purging, Type I converts to Type B.

FIG. 58 shows thermo-gravimetric analysis (TGA) plot of crystalline Type I of Formula IX.

FIG. 59 shows an X-ray powder diffraction (XRPD) pattern of crystalline Type J of Formula IX.

FIG. 60 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of crystalline Type J of Formula IX.

FIG. 61 shows ¹H NMR spectrum of crystalline Type J of Formula IX.

FIG. 62 shows X-ray powder diffraction (XRPD) patterns of crystalline Type J of Formula IX before heating (top), heating to 130° C. (second from top), crystalline Type A reference (second from bottom), and crystalline Type I reference (bottom). Upon heating, Type J converts to a mixture of Type A and Type I.

FIG. 63 shows an X-ray powder diffraction (XRPD) pattern of crystalline Type K of Formula IX.

FIG. 64 shows thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC) plots of crystalline Type K of Formula IX.

DETAILED DESCRIPTION I. General

The present disclosure, in part, provides an improved process for the preparation of a compound of Formula IX (AG-10) and intermediates thereof. The newly described process provides high yields and improved efficiency.

While a complete synthetic scheme is provided in the summary of the invention, as well as Scheme 1 (FIG. 1), one of skill in the art will appreciate that selected steps of the instant process are novel and can be performed independent of the origin of the starting material or intermediates.

Also provided herein is a pharmaceutically acceptable salt of Formula I and Formula Ib. Pharmaceutically acceptable salts of Formula I and Formula Ib possess surprising pharmacokinetic properties which improves the bioavailability of the compound of Formula IX. Without being bound to any particular theory, it is believed that the pharmaceutically acceptable salt of Formula I and Formula Ib provide a protonated pyrazole on the compound of formula IX that pairs with the anion of the protic acid or multiprotic acid. Unlike pharmaceutically acceptable salts of Formula I and Formula Ib, salts prepared from alkali hydroxides, such as NaOH, or the zwitterion of the compound of Formula DC do not provide the advantageous features described herein. In particular embodiments the compound of formula I is represented by the compound of Formula Ia, the HCl salt of Formula I.

II. Definitions

The term “compound of Formula IX” refers to 3-(3-(3,5-dimethyl-1H-pyrazol-4-yl)propoxy)-4-fluorobenzoic acid, also known as AG-10, a compound with the following structure

The terms “a,” “an,” or “the” as used herein not only include aspects with one member, but also include aspects with more than one member. For instance, the singular forms “a,” “an,” and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to “a cell” includes a plurality of such cells and reference to “the agent” includes reference to one or more agents known to those skilled in the art, and so forth.

The term “alkyl” refers to a straight or branched, saturated, aliphatic radical having the number of carbon atoms indicated. Alkyl can include any number of carbons, such as C₁₋₂, C₁₋₃, C₁₋₄, C₁₋₅, C₁₋₆, C₁₋₇, C₁₋₈, C₁₋₉, C₁₋₁₀, C₂₋₃, C₂₋₄, C₂₋₅, C₂₋₆, C₃₋₄, C₃₋₅, C₃₋₆, C₄₋₅, C₄₋₆ and C₅₋₆. For example, C₁₋₆ alkyl includes, but is not limited to, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, isopentyl, hexyl, etc. Alkyl can also refer to alkyl groups having up to 20 carbons atoms, such as, but not limited to heptyl, octyl, nonyl, decyl, etc. Alkyl groups can be substituted or unsubstituted. Particular substituents include, hydroxyl, halogen, alkoxy and amino groups. A person of skill in the art will recognize that a number of substituents may be added to alkyl groups without departing from the teachings herein,

The term “alkenyl” refers to a straight chain or branched hydrocarbon having at least 2 carbon atoms and at least one double bond. Alkenyl can include any number of carbons, such as C₂, C₂₋₃, C₂₋₄, C₂₋₅, C₂₋₆, C₂₋₇, C₂₋₈, C₂₋₉, C₂₋₁₀, C₃, C₃₋₄, C₃₋₅, C₃₋₆, C₄, C₄₋₅, C₄₋₆, C₅, C₅₋₆, and C₆. Alkenyl groups can have any suitable number of double bonds, including, but not limited to, 1, 2, 3, 4, 5 or more. Examples of alkenyl groups include, but are not limited to, vinyl (ethenyl), propenyl, isopropenyl, 1-butenyl, 2-butenyl, isobutenyl, butadienyl, 1-pentenyl, 2-pentenyl, isopentenyl, 1,3-pentadienyl, 1,4-pentadienyl, 1-hexenyl, 2-hexenyl, 3-hexenyl, 1,3-hexadienyl, 1,4-hexadienyl, 1,5-hexadienyl, 2,4-hexadienyl, or 1,3,5-hexatrienyl. Alkenyl groups, like the alkyl groups describe above, can be substituted or unsubstituted.

The term “alkynyl” refers to either a straight chain or branched hydrocarbon having at least 2 carbon atoms and at least one triple bond. Alkynyl can include any number of carbons, such as C₂, C₂₋₃, C₂₄, C₂₋₅, C₂₋₆, C₂₋₇, C₂₋₈, C₂₋₉, C₂₋₁₀, C₃, C₃₄, C₃₋₅, C₃₋₆, C₄, C₄₋₅, C₄₋₆, C₅, C₅₋₆, and C₆. Examples of alkynyl groups include, but are not limited to, acetylenyl, propynyl, 1-butynyl, 2-butynyl, isobutynyl, sec-butynyl, butadiynyl, 1-pentynyl, 2-pentynyl, isopentynyl, 1,3-pentadiynyl, 1,4-pentadiynyl, 1-hexynyl, 2-hexynyl, 3-hexynyl, 1,3-hexadiynyl, 1,4-hexadiynyl, 1,5-hexadiynyl, 2,4-hexadiynyl, or 1,3,5-hexatriynyl. Alkynyl groups, like the alkyl groups describe above, can be substituted or unsubstituted.

The term “cycloalkyl” refers to a saturated or partially unsaturated, monocyclic, fused bicyclic or bridged polycyclic ring assembly containing from 3 to 12 ring atoms, or the number of atoms indicated. Cycloalkyl can include any number of carbons, such as C₃₋₆, C₄₋₆, C₅₋₆, C₃₋₈, C₄₋₈, C₅₋₈, C₆₋₈, C₃₋₉, C₃₋₁₀, C₃₋₁₁, and C_(3-12.) Saturated monocyclic cycloalkyl rings include, for example, cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl. Cycloalkyl groups can also be partially unsaturated, having one or more double or triple bonds in the ring. When cycloalkyl is a saturated monocyclic C₃₋₈ cycloalkyl, exemplary groups include, but are not limited to cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and cyclooctyl. Cycloalkyl groups can be substituted or unsubstituted. As a person of skill in the art will recognize, many different substituents of cycloalkyl groups can be included without departing from the teachings herein.

The term “heterocycloalkyl” refers to a saturated ring system having from 3 to 12 ring members and from 1 to 4 heteroatoms of N, 0 and S. Additional heteroatoms can also be useful, including, but not limited to, B, Al, Si and P. The heteroatoms can also be oxidized, such as, but not limited to, —S(O)— and —S(O)₂—. Heterocycloalkyl groups can include any number of ring atoms, such as, 3 to 6, 4 to 6, 5 to 6, 3 to 8, 4 to 8, 5 to 8, 6 to 8, 3 to 9, 3 to 10, 3 to 11, or 3 to 12 ring members. Any suitable number of heteroatoms can be included in the heterocycloalkyl groups, such as 1, 2, 3, or 4, or 1 to 2, 1 to 3, 1 to 4, 2 to 3, 2 to 4, or 3 to 4. Heterocycloalkyl groups, like the cycloalkyl groups describe above, can be substituted or unsubstituted.

The term “aryl” refers to an aromatic ring system having any suitable number of ring atoms and any suitable number of rings. Aryl groups can include any suitable number of ring atoms, such as, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or 16 ring atoms, as well as from 6 to 10, 6 to 12, or 6 to 14 ring members. Aryl groups can be monocyclic, fused to form bicyclic or tricyclic groups, or linked by a bond to form a biaryl group. Representative aryl groups include phenyl, naphthyl and biphenyl. Other aryl groups include benzyl, having a methylene linking group. Some aryl groups have from 6 to 12 ring members, such as phenyl, naphthyl or biphenyl. Other aryl groups have from 6 to 10 ring members, such as phenyl or naphthyl. Some other aryl groups have 6 ring members, such as phenyl. Aryl groups, like the cycloalkyl groups describe above, can be substituted or unsubstituted.

The term “heteroaryl” refers to a monocyclic or fused bicyclic or tricyclic aromatic ring assembly containing 5 to 16 ring atoms, where from 1 to 5 of the ring atoms are a heteroatom such as N, O or S. Additional heteroatoms can also be useful, including, but not limited to, B, Al, Si and P. The heteroatoms can also be oxidized, such as, but not limited to, —S(O)— and —S(O)₂—. Heteroaryl groups can include any number of ring atoms, such as, 3 to 6, 4 to 6, 5 to 6, 3 to 8, 4 to 8, 5 to 8, 6 to 8, 3 to 9, 3 to 10, 3 to 11, or 3 to 12 ring members. Any suitable number of heteroatoms can be included in the heteroaryl groups, such as 1, 2, 3, 4, or 5, or 1 to 2, 1 to 3, 1 to 4, 1 to 5, 2 to 3, 2 to 4, 2 to 5, 3 to 4, or 3 to 5. Heteroaryl groups can have from 5 to 8 ring members and from 1 to 4 heteroatoms, or from 5 to 8 ring members and from 1 to 3 heteroatoms, or from 5 to 6 ring members and from 1 to 4 heteroatoms, or from 5 to 6 ring members and from 1 to 3 heteroatoms. The heteroaryl group can include groups such as pyrrole, pyridine, imidazole, pyrazole, triazole, tetrazole, pyrazine, pyrimidine, pyridazine, triazine thiophene, furan, thiazole, isothiazole, oxazole, and isoxazole. Heteroaryl groups, like the cycloalkyl groups describe above, can be substituted or unsubstituted.

The term “halogen” refers to fluorine, chlorine, bromine and iodine.

The term “hydrated” refers to a chemical reagent that contains water. Hydrated, in the context of the chemical conversion of step (a) refers to a chemical reagent with a sufficient amount of water to complete the chemical conversion shown. In particular embodiments, a hydrated reagent includes at least 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, or 20% by weight water content.

III. EMBODIMENTS OF THE DISCLOSURE

A. Process for Preparing a Compound of Formula IX

In one aspect, the present disclosure provides an improved method for prepairing a compound of Formula IX

comprising

-   -   (a) contacting a compound of Formula II

-   -   with a compound of Formula III

-   -   a first base, and a first organic solvent to provide a compound         of Formula IV

-   -   wherein each R¹ is independently a halogen or a sulfonate ester;     -   (b) contacting a compound of Formula IV with hydrazine and a         second organic solvent to provide a compound of Formula V

-   -   (c) contacting a compound of Formula V with a sulfonating agent         or halogenating agent provide a compound of Formula VI

-   -   wherein R² is a halogen or a sulfonate ester;     -   (d) contacting a compound of Formula VI with a compound of         Formula VII

-   -   a second base, and an third organic solvent to provide a         compound of Formula VIII

-   -   wherein R³ is selected from the group consisting of an C₁₋₁₂         alkyl, C₂₋₁₂ alkenyl, C₁₋₁₂ alkynyl, Cmcycloalkyl,         heterocycloalkyl, aryl, and heteroaryl, each of which is         optionally substituted.     -   (e) contacting a compound of Formula VIII with a third base to         provide a compound of Formula IX.

Step (a), comprises contacting a first base and an organic solvent with a compound of Formula II

and a compound of Formula III

to provide a compound of Formula IV

A person of skill in the art will recognize that the compound of Formula IV has both nucleophilic and electrophilic sites and that depending on the reaction conditions intramolecular conversions are possible. For example, under some conditions, the alcohol group of Formula IV may add to one of the carbonyl carbons to form a six membered ring (Formula IVa). When the afore mentioned addition is coupled with a subsequent elimination reaction, the compound of Formula IV has the structure of Formula IVb. It is further apparent to a person of skill in the art that the compound of Formula IV can also exist as the enol tautomer of Formula IVc.

The compounds of Formula IV, Formula IVa, Formula IVb, Formula IVc interconvert, and depending on the reaction conditions varying concentrations of these species are present. In some embodiments, only a single species is present.

There are many suitable bases which may be used in this conversion. For example, in some embodiments, the first base is an alkali metal carbonate, an alkali metal bicarbonate or a combination thereof. Alkali metal carbonates can include, but are not limited to Li₂CO₃, Na2CO₃, and K₂CO₃′ and alkali metal bicarbonates can include, but are not limited to LiHCO₃, NaHCO₃, and KHCO₃. In some embodiments, the alkali metal carbonate is K₂CO₃.

The organic solvent of step (a) is one which will suitably dissolve both compounds of Formula II and Formula III in solution, and be miscible with the base being used. A person of skill in the art will recognize that there are a number of organic solvents which meet these specifications. In some embodiments, the first organic solvent is a polar organic solvent. In some embodiments, the polar organic solvent is selected from the group consisting of acetone, ethyl acetate, dichloromethane, tetrahydrofuran, dimethylformamide and acetonitrile. In some embodiments, the organic solvent is acetone.

The conversion of step (a) also includes at least 1 equivalent of water to produce the hydroxyl containing compound of Formula IV. Often, this equivalent of water is provided by the reagents or solvents in the reaction, such as the first base or the organic solvent, rather than the direct addition of water. It has been found that the use of hydrated base in the chemical conversion of step (a) provides an exceptionally efficient conversion. As such, in some embodiments, the first base in the conversion of step (a) is a hydrated base. In some embodiments, the first base is a hydrated alkali metal carbonate. In some embodiments, the first base is a hydrated K₂CO₃.

The compound of Formula III includes two R¹ groups, each of which are independently selected from the group consisting of chloride, bromide, tosylate, and mesylate.

In some embodiments, each R¹ is bromide.

It can be seen that each R¹ group acts as a leaving group in the conversion in step (a); thus, a person of skill in the art will recognize that other leaving groups are useful in the present invention and do not depart from the teachings herein.

In some embodiments, the compound of Formula IV provided in step (a) is used directly in the conversion of step (b) without purification.

Turning to step (b), the chemical conversion described comprises a compound of Formula IV

with hydrazine (N₂H₄) and a second organic solvent to provide a compound of Formula V

A person of skill in the art will recognize that a variety of different solvents can be used as the second organic solvent in this conversion. In some embodiments, the second organic solvent is a polar protic organic solvent. In some embodiments, the polar protic organic solvent is C₁₋₈—OH. In some embodiments, the polar protic organic solvent is ethanol.

In some embodiments, the compound of Formula V provided in step (b) is used directly in the conversion of step (c) without purification.

The chemical conversion of step (c) includes the replacement of the hydroxyl moiety in the compound of Formula (V) with halogen or the conversion of the hydroxyl to a sulfonate ester to provide a compound of Formula (VI)

Although sulfonating agents or halogenating agents are particularly envisioned as starting materials in this conversion, a person of skill in the art will recognized that many different leaving groups beyond halogens and sulfate esters are appropriate substituents for R². Thus, any starting material that will yield a suitable leaving group at the R² position is within the scope of this invention.

There are a number of solvents which are suitable for this conversion; however, a person of skill in the art will recognize that the solvent chosen for this chemical conversion will depend upon the sulfonating agent or halogenating agent chosen, as particular solvents might not be suitable for all starting materials. For example, when contacting the compound of Formula V with a halogenating agent, polar organic solvents are particularly useful. In some embodiments, the polar organic solvent is 1,2-dichloroethane.

Halogenating agents useful in the conversion of step (c) include, but are not limited to, PBr₃, PCl₃, PCl₅, SOBr₂, PBr₅, and SoCl₂. Sulfonating agents of the conversion of step (c) include, but are not limited to, mesyl chloride (MsCl) and tosyl chloride (TsCl). In some embodiments, the halogenating agent is PBr₃.

It is understood that the identity of R² is dependent upon the starting material chosen for the chemical conversion in step (c). For example, if a sulfonating agent is chosen, the identity of R² is the corresponding sulfate. In some embodiments, R² is chloride, bromide, tosylate, and mesylate. In some embodiments, R² is Br.

Focusing on step (d), the compounds of Formula VI

and Formula VII

are contacted in the presence of a base and a third organic solvent to provide a compound of Formula VIII

The chemical conversion of step (d) can be performed with a variety of different bases. For example, in some embodiments, the second base is an alkali metal carbonate, an alkali metal bicarbonate or a combination thereof. Alkali metal carbonates can include, but are not limited to Li₂CO₃, Na₂CO₃, and K₂CO₃′ and alkali metal bicarbonates can include, but are not limited to LiHCO₃, NaHCO₃, and KHCO₃. In some embodiments, the alkali metal carbonate is K₂CO₃.

The organic solvent of step (d) is one which will suitably dissolve both compounds of Formula VI and Formula VII in solution, and be miscible with the base being used. A person of skill in the art will recognize that there are a number of organic solvents which meet these specifications. In some embodiments, the third organic solvent is a polar aprotic organic solvent. In some embodiments, the polar organic solvent is selected from the group consisting of acetone, ethyl acetate, dichloromethane, tetrahydrofuran, dimethylformamide, dimethyl sulfoxide, and acetonitrile. In some embodiments, the third organic solvent is dimethylformamide. In some embodiments, the third organic solvent is dimethyl sulfoxide.

Suitable substituents for the R³ group include those which will not interfere with the chemical conversion of step (e), discussed in more detail below. Such substituents, include, but are not limited to C₁₋₈alkyl, C₃₋₈ cycloalkyl, C₃₋₁₂heterocycloalkyl, aryl, heteroaryl, etc. A person of skill in the art will recognize that many other ester substituents of R³ are suitable without departing from the teachings herein. In some embodiments, R³ is C₁₋₈alkyl. In some embodiments, R³ is methyl.

In some embodiments, the process of step (d) provides a compound of Formula VIII with at least a 70% yield (mol/mol) relative to the amount of Formula VII.

With respect to step (e), a compound of Formula VIII

is contacted with a third base to provide a compound of Formula IX

The third base in the chemical conversion of step (e) can be a number of different bases. For example, in some embodiments, the third base is a metal hydroxide. In some embodiments, the metal hydroxide is an alkali metal hydroxide. In some embodiments, the alkali metal hydroxide is a selected from the group consisting of LiOH, NaOH, KOH, RbOH, and CsOH. In some embodiments, the alkali metal hydroxide is LiOH. In some embodiments, the alkali metal hydroxide is NaOH.

A person of skill in the art will recognize that a variety of different solvents can be used as the solvent in conversion of step (e). For instance, in some embodiments, the second organic solvent is a polar protic organic solvent or water. In some embodiments, the polar protic organic solvent is C₁₋₈—OH. In some embodiments, the polar protic organic solvent is methanol. In some embodiments, the solvent is water. In some embodiments, the solvent is a combination of methanol and water.

In some embodiments, the process in step (e) further comprises

(e-i) removing the solvent to provide a residue;

(e-ii) dissolving the residue in water to form a solution;

(e-iii) acidifying the solution to form a precipitate; and

(e-iv) filtering the solution to provide isolated Formula IX,

wherein steps (e-i) to (e-iv) is performed after step (e).

Step (e-i) may be performed using any suitable removal step such as reduced pressure, temperature elevation, or a combination of both. In some embodiments, the solvent is removed under reduced pressure. In some embodiments, a solid is produced in step (e) and the solvent is removed via filtration. Further, the addition of water in step (e-ii) can be performed prior to step (e-i). In such event, the removal of the solvent via reduced pressure provides a concentrated aqueous component (i.e. the water is not removed). It is understood that the re-ordering of steps (e-i) and (e-ii) does not depart from the processes described herein.

Step (e-iii) may be acidified with any suitable acid. In some embodiments, the suitable acid is HCl. In some embodiments, the solution is acidified to a pH of below 3, 0-3, or 2-3. In some embodiments the solution is acidified to a solution of about 2. In some embodiments, the solution is acidified to a pH of below 2, 0-2, or 1-2. In some embodiments, the solution is acidified to a pH of about 1.4-1.6.

The pH of the acidifying step (e-iii) determines the predominant species produced. In some embodiments, the pH of the acidifying step is in the range of 5-6 and the zwitterionic form of Formula IX is produced. In some embodiments, the pH is acidified with HCl to less than about 2 or in the range of 1.4 to 1.6 and the HCl salt of Formula IX is produced (i.e. the compound of Formula Ia).

The process described in step (e) can produce a compound of Formula IX with high yield and purity. In some embodiments, the yield of step (e) is greater than 85%, 90%, 93%, or 95% (mol/mol) relative to Formula VIII. In some embodiments the purity of the compound of Formula IX produced in step (e) is greater than 80%, 85%, 90%, 95%, or 97% pure (mol/mol).

In another aspect, provided herein is a method of prepairing a compound of Formula IX

comprising

-   -   (a) contacting a compound of Formula II

-   -   with a compound of Formula III

-   -   a first base, and a first organic solvent to provide an adduct         -   wherein each R¹ is independently a halogen or a sulfonate             ester;     -   (b) contacting the adduct with hydrazine and a second organic         solvent to provide a compound of Formula V

-   -   (c) contacting a compound of Formula V with a sulfonating agent         or halogenating agent provide a compound of Formula VI

-   -   wherein R² is a halogen or a sulfonate ester;     -   (d) contacting a compound of Formula VI with a compound of         Formula VII

-   -   a second base, and an third organic solvent to provide a         compound of Formula VIII

-   -   wherein R³ is selected from the group consisting of an C₁₋₁₂         alkyl, C₂₋₁₂ alkenyl, C₁₋₁₂ alkynyl, C₃₋₈cycloalkyl,         heterocycloalkyl, aryl, and heteroaryl, each of which is         optionally substituted.     -   (e) contacting a compound of Formula VIII with a third base to         provide a compound of Formula IX.

In some embodiments, the adduct produced in step (a) is a compound of Formula IV, Formula IVa, Formula IVb, and/or Formula IVc.

A person of skill in the art will recognize that the compounds shown above can interconvert and that the relative amounts of each compound are dependent on the experimental conditions.

As noted above, a person of skill in the art will appreciate that selected steps in the process may be conducted independent of the origin of starting material or intermediates.

B. Pharmaceutically Acceptable Salts of Formula I

In a second aspect, the present disclosure provides a phamraceutically acceptable salt respresented by Formula I

wherein X is a pharmaceutically acceptable anion of a protic acid.

A variety of protic acids are suitable for making a pharmaceutically acceptable salt of Formula I. It can be seen that the pharmaceutically acceptable anion of the protic acid is dependent upon the protic acid used. For example, protic acids useful in the present disclosure include hydrochloric acid, hydrobromic acid, sulfonic acid, tosylic acid (p-toluenesulfonic acid), methanesulfonic acid, nitric acid, or acetic acid. Thus, pharmaceutically acceptable anions of a protic acid include chloride (Cl⁻), bromide (Br), sulfonate (HS(O)₂O⁻), tosylate (TsO⁻), mesylate (MsO⁻), besylate (BeO⁻), ethanesulfonate (EtSO₃ ⁻), nitrate (NO₃ ⁻), acetate (CH₃C(O)O⁻), glycolate (HO—CH₂—C(O)O⁻), or combinations thereof.

In some embodiments, the pharmaceutically acceptable anion of a protic acid is mesylate. In some embodiments, the mesylate salt of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG. 4.

In some embodiments, the pharmaceutically acceptable anion of a protic acid is besylate. In some embodiments, the mesylate salt of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG. 8.

In some embodiments, the pharmaceutically acceptable anion of a protic acid is tosylate. In some embodiments, the tosylate salt of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG. 10.

In some embodiments, the pharmaceutically acceptable anion of a protic acid is esylate. In some embodiments, the esylate salt of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG. 12.

In some embodiments, the pharmaceutically acceptable anion of a protic acid is bromide. In some embodiments, the bromide salt of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG. 14.

In some embodiments, the pharmaceutically acceptable anion of a protic acid is nitrate. In some embodiments, the nitrate salt is characterized by an XRPD pattern substantially in accordance with FIG. 16. In some embodiments, the nitrate salt is characterized by an XRPD pattern substantially in accordance with FIG. 18.

In some embodiments, the pharmaceutically acceptable anion of a protic acid is chloride, and the pharmaceutically acceptable salt of Formula I is represented by Formula (Ia)

In some embodiments, the salt of Formula Ia is crystalline Type A. In some embodiments, crystalline Type A of Formula Ia is characterized by an X-ray powder diffraction pattern comprising peaks at 12.0, 21.8, 25.9, 26.7, and 27.9 degrees 2θ (±0.2 degrees 2θ). In some embodiments, the X-ray powder diffraction pattern further comprises one or more peaks at 7.0, 10.3, 13.9, 15.6, and/or 17. Crystalline Type A of Formula Ia characterized by an X-ray powder diffraction pattern substantially in accordance with FIG. 31.

In some embodiments, a multiprotic acid, such as a diprotic or triprotic acid, are used to make pharmaceutically acceptable salts of Formula IX. In such embodiments, the pliaimaceutically acceptable salt is represented by Formula Ib

where Y is a multiprotic acid.

In some embodiments, Y is selected from the group consisting of ethane-1,2,-disulfonic acid, sulfuric acid, citric acid, maleic acid, malic acid, tartaric acid, and oxalic acid. In some embodiments, Y is L-malic acid or L-tartaric acid.

In some embodiments Y is ethane-1,2,-disulfonic acid. In some embodiments, the edisylate salt of Formula IX is characterized by an XRPD pattern substantially in accordance with FIG. 6.

In some embodiments Y is sulfuric acid. In some embodiments, the sulfate salt of Formula IX is characterized by an XRPD pattern substantially in accordance with FIG. 19.

In some embodiments Y is oxalic acid. In some embodiments, the oxalate salt of Formula IX is characterized by an XRPD pattern substantially in accordance with FIG. 21.

In some embodiments Y is maleic acid. In some embodiments, the maleate salt of Formula IX is characterized by an XRPD pattern substantially in accordance with FIG. 23. In some embodiments, the maleate salt of Formula IX is characterized by an XRPD pattern substantially in accordance with FIG. 25.

In some embodiments Y is acetic acid. In some embodiments, the acetic acid salt of Formula IX is characterized by an XRPD pattern substantially in accordance with FIG. 27.

In some embodiments Y is L-malic acid. In some embodiments, the L-malic acid salt of Formula IX is characterized by an XRPD pattern substantially in accordance with FIG. 29.

The molar ratios of AG-10 and Y in Formula Ib can vary depending on the multiprotic acid used. For example, when Y is maleic acid, the molar ratio of AG-10 to Y is 1:1; when Y is edisylate, the molar ratio of AG-10 to Y is 2:1; and when Y is malic acid, the molar ratio of AG-10 to Y is 1.8:1.

Pharmaceutically acceptable salts of Formula I can be produced using a number of conventional means in the art. For example, the free acid form of a compound of Formula I may be contacted with a stoichiometric amount of the appropriate acid in water, an organic solvent, or a mixture of the two. In some embodiments, pharmaceutically acceptable salts of Formula I are made in nonaqueous media such as an ether, ethyl acetate, ethanol, isopropanol, or acetonitrile. In some embodiments, the pharmaceutically acceptable salts of Formula I are made by dissolving a compound of Formula IX in water, adding a suitable amount of HX to form a mixture, and adding a nonaqueous solvent, such as the nonaqueous media described above to crystallize the salt. In some embodiments, a suitable amount of HX is a stoichiometric amount. It is understood the HX comprises a hydrogen and an X is a pharmaceutically acceptable anion of a protic acid as defined above.

As with pharmaceutically acceptable salts of Formula I, pharmaceutically acceptable salts of Formula Ib can also be produced using a number of conventional means in the art. As a non-limiting example, the free acid form of a compound of Formula Ib may be contacted with a stoichiometric or sub-stoichiometric amount of the appropriate multiprotic acid in water, an organic solvent, or a mixture of the two to produce a pharmaceutically acceptable salt of Formula Ib.

C. Crystalline Forms of Formula IX

In a further aspect, provided herein are crystalline forms of Formula IX

The current disclosure describes eleven crystalline forms of Formula IX, six HCl salt forms (Type A, Type B, Type E, Type H, Type I, and Type J), three free base forms (Type K, Type C and Type G), and two unidentified forms (Type D and Type F). A summary of the properties of the identified forms are provided in Table 1 and Table 2.

In some embodiments, the crystalline forms of Formula IX provided herein are substantially free of other crystalline forms. The term “substantially free” refers to an amount of 10% or less of another form, preferably 8%, 5%, 4%, 3%, 2%, 1%, 0.5%, or less of another form.

TABLE 1 Characterization results of HCl salt crystal forms HPLC Stoichi- Crystal Form Weight Endotherm Purity ometry Speculated Batch No. Loss % (peak, ° C.) (area %) (acid:FB)* Form Type A 1.3 111.7, 212.6, 98.76 0.91 237.3 Type B 1.2 161.4, 232.2, 97.86 0.86 Anhydrate 262.3 Type E 1.5 182.0, 242.7  98.60 0.91 Type I 3.0  62.0, 158.4, 97.94 0.86 Hydrate 215.7 Type H 4.6  90.4, 200.5, 98.47 0.91 MeOH 232.3 solvate Type J 21.5 120.8, 197.8, 91.69 0.90 DMAc 221.5 solvate *the Stoichiometry was determined by HPLC/IC using freebase Type A (TRM-01658-2) as the standard sample. A routine method was used and no systemic development and validation was conducted.

TABLE 2 Characterization results of freebase forms Crystal Form Weight Endotherm HPLC Purity Cl⁻ Content Batch No. Loss % (peak, ° C.) (area %) (%)* Type K 6.1 159.3, 176.2, 99.12 — (monohydrate) 278.4 Type C 3.1  91.2, 173.0 — 0.17 Type G 3.7 231.1 99.46 0.14 —: no data available. *the theoretical Cl⁻ content of mono-HCl salt is 10.8%. Based on the results of Cl⁻ content, Type C and G were determined to be freebase forms.

In some embodiments, provided herein is crystalline Type A of Formula IX. In some embodiments, crystalline Type A of Formula IX is characterized by an X-ray powder diffraction pattern comprising peaks at 7.0, 10.4, 12.0, 13.0, and 13.9 degrees 2θ (±0.2 degrees 2θ). In some embodiments, crystalline Type A of Formula IX is characterized by an X-ray powder diffraction pattern comprising peaks at 12.0, 21.8, 25.9, 26.7, and 27.9 degrees 2θ (±0.2 degrees 2θ). In some embodiments, the X-ray powder diffraction pattern further comprises one or more peaks at 7.0, 10.3, 13.9, 15.6, and/or 17. In some embodiments, crystalline Type A of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG. 31. In some embodiments crystalline Type A of Formula IX is substantially free of other crystalline forms.

In some embodiments, crystalline Type A of Formula IX is characterized by a weight loss ranging from about 0.7% to about 1.9% upon heating to around 150° C., as measured by thermal gravimetric analysis. In some embodiments, the weight loss is about 1.3% as measured by thermal gravimetric analysis.

In some embodiments, Crystalline Type A of Formula IX is characterized by water uptake of about 1.6% at 25° C./80% relative humidity (RH) after undergoing a dynamic vapor sorption cycle which includes pre-equilibration at 0% RH. In some embodiments, crystalline Type A of Formula IX characterized by gains of less than 2.5% weight after undergoing a dynamic vapor sorption cycle from about 0% relative humidity (RH) to about 90% RH. In some embodiments, crystalline Type A of Formula IX has a dynamic vapor sorption profile substantially as shown in FIG. 37.

In some embodiments, crystalline Type A of Formula IX is characterized by a differential scanning calornnetry thermogram comprising endothermic peaks at around 211-214 and 237-239° C. In some embodiments, the differential scanning calorimetry thermogram comprises endothermic peaks around 11.7, 212.6, and 237.3° C.

In some embodiments, provided herein is crystalline Type B of Formula IX. In some embodiments, crystalline Type B of Formula IX is characterized by an X-ray powder diffraction pattern comprising peaks at 12.0, 13.8, 17.2, 17.7, and 19.8 degrees 20 (±0.2 degrees 20). In some embodiments, crystalline Type B of Formula IX characterized by an X-ray powder diffraction pattern comprising peaks at 12.1, 13.9, 19.8, 23.3, and 24.4 degrees 2θ (±0.2 degrees 2θ). In some embodiments, crystalline Type B of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG. 40. In some embodiments crystalline Type B of Formula IX is substantially free of other crystalline forms.

In some embodiments, crystalline Type B of Formula IX is characterized by a weight loss from about 0.6% to about 2.0% upon heating to around 150° C., as measured by thermal gravimetric analysis. In some embodiments, crystalline Type B of Formula IX is characterized by a weight loss of about 1.2% upon heating to around 150° C., as measured by thermal gravimetric analysis.

In some embodiments, crystalline Type B of Formula IX is characterized by a differential scanning calorimetry thermogram comprising endothermic peaks at around 161.4, 232.2 and 262.3° C.

In some embodiments, provided herein is crystalline Type E of Formula IX. In some embodiments, crystalline Type E of Formula IX is characterized by an X-ray powder diffraction pattern comprising peaks at 11.8, 14.0, 15.1, 19.9, and 24.0 degrees 2θ (±0.2 degrees 2θ). In some embodiments, crystalline Type E of Formula IX characterized by an X-ray powder diffraction pattern comprising peaks at 11.9, 14.0, 15.1, and 25.8 degrees 20 (±0.2 degrees 20). In some embodiments, crystalline Type E of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG. 46. In some embodiments crystalline Type E of Formula IX is substantially free of other crystalline forms.

In some embodiments, crystalline Type E of Formula IX is characterized by a weight loss from about 0.5% to about 2.5% upon heating to around 150° C., as measured by thermal gravimctric analysis. In some embodiments, crystalline Type E of Formula IX is characterized by a weight loss of about 1.5% upon heating to around 150° C., as measured by thermal gravimetric analysis.

In some embodiments, crystalline Type E of Formula IX is characterized by a differential scanning calorimetry thermogram comprising endothermic peaks at around 182.0 and 242.7° C.

In some embodiments, provided herein is crystalline Type I of Formula IX. In some embodiments, crystalline Type I of Formula IX is characterized by an X-ray powder diffraction pattern comprising peaks at 11.4, 12.1, 12.4, 13.6, and 13.9 degrees 2θ (±0.2 degrees 2θ). In some embodiments, crystalline Type I of Formula IX characterized by an X-ray powder diffraction pattern comprising peaks at 12.5, 17.3, 23.4, 25.0, and 25.4 degrees 2θ (±0.2 degrees 2θ). In some embodiments, crystalline Type I of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG. 55. In some embodiments crystalline Type I of Formula IX is substantially free of other crystalline forms.

In some embodiments, crystalline Type I of Formula IX is characterized by a weight loss ranging from about 2.5% to 3.5% upon heating to around 120° C., as measured by thermal gravimetric analysis. In some embodiments, crystalline Type I of Formula IX is characterized by a weight loss of about 3.0% upon heating to around 120° C., as measured by thermal gravimetric analysis.

In some embodiments, crystalline Type I of Formula IX is characterized by a differential scanning calorimetry thermogram comprising endothermic peaks at around 62.0 and 158.4, 215.7° C.

In some embodiments, provided herein is crystalline Type H of Formula IX. In some embodiments, crystalline Type H of Formula IX is characterized by an X-ray powder diffraction pattern comprising peaks at 11.8, 12.3, 13.8, 15.7, and 16.9 degrees 2θ (±0.2 degrees 2θ). In some embodiments, Crystalline Type H of Formula IX characterized by an X-ray powder diffraction pattern comprising peaks at 11.9, 12.3, 21.7, 23.3, and 25.8 degrees 2θ (±0.2 degrees 2θ). In some embodiments, crystalline Type H of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG. 51. In some embodiments crystalline Type H of Formula IX is substantially free of other crystalline forms.

In some embodiments, Crystalline Type H of Formula IX is characterized by a weight loss ranging from about 3.5% to about 5.5% upon heating to around 150° C., as measured by thermal gravimetric analysis. In some embodiments, crystalline Type H of Formula IX is characterized by a weight loss of about 4.6% upon heating to around 150° C., as measured by thermal gravimetric analysis.

In some embodiments, crystalline Type H of Formula IX is characterized by a differential scanning calorimetry thermogram comprising endothermic peaks at around 90.4 and 200.5, 232.3° C.

In some embodiments, provided herein is crystalline Type J of Formula IX. In some embodiments, crystalline Type J of Formula IX is characterized by an X-ray powder diffraction pattern comprising peaks at 4.6, 11.8, 12.8, 13.8, and 14.6 degrees 2θ (±0.2 degrees 2θ). In some embodiments, Crystalline Type J of Formula IX characterized by an X-ray powder diffraction pattern comprising peaks at 13.8, 14.7, 22.9, 26.2, and 27.7 degrees 2θ (±0.2 degrees 2θ). In some embodiments, crystalline Type J of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG. 59. In some embodiments crystalline Type J of Formula IX is substantially free of other crystalline forms.

In some embodiments, crystalline Type J of Formula IX is characterized by a weight loss ranging from about 17.5% to about 24% upon heating to around 120° C., as measured by thermal gravimetric analysis. In some embodiments, crystalline Type J of Formula IX is characterized by a weight loss of about 21.5% upon heating to around 120° C., as measured by thermal gravimetric analysis.

In some embodiments, crystalline Type J of Formula IX is characterized by a differential scanning calorimetry thermogram comprising endothermic peaks at around 120.8 and 197.8, 221.5° C.

In some embodiments, provided herein is crystalline Type K of Formula IX. In some embodiments, crystalline Type K of Formula IX is characterized by an X-ray powder diffraction pattern comprising peaks at 7.5 9.8, 13.9, 15.9, and 19.3 degrees 2θ (±0.2 degrees 2θ). In some embodiments, crystalline Type K of Formula IX characterized by an X-ray powder diffraction pattern comprising peaks at 7.2, 7.6, 9.9, 14.0, and 19.3 degrees 20 (±0.2 degrees 20). In some embodiments, crystalline Type K of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG. 59. In some embodiments crystalline Type K of Formula IX is substantially free of other crystalline forms.

In some embodiments, crystalline Type K of Formula IX is characterized by a weight loss ranging from about 5.0% to about 7.0% upon heating to around 120° C., as measured by thermal gravimetric analysis. In some embodiments, crystalline Type K of Formula IX is characterized by a weight loss of about 6.1% upon heating to around 120° C., as measured by thermal gravimetric analysis.

In some embodiments, crystalline Type K of Formula IX is characterized by a differential scanning calorimetry thermogram comprising endothermic peaks at around 159.3 and 176.2, 278.4° C.

In some embodiments, provided herein is crystalline Type C of Formula IX. In some embodiments, crystalline Type C of Formula IX is characterized by an X-ray powder diffraction pattern comprising peaks at 9.5, 11.7, 12.3, 13.4, and 14.6 degrees 2θ (±0.2 degrees 2θ). In some embodiments, Crystalline Type C of Formula IX characterized by an X-ray powder diffraction pattern comprising peaks at 14.6, 16.8, 19.5, 20.7, and 22.5 degrees 2θ (±0.2 degrees 2θ). In some embodiments, crystalline Type C of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG. 43. In some embodiments crystalline Type C of Formula IX is substantially free of other crystalline forms.

In some embodiments, crystalline Type C of Formula IX is characterized by a weight loss ranging from about 2.0% to about 4.0% upon heating to around 150° C., as measured by thermal gravimetric analysis. In some embodiments, crystalline Type C of Formula IX is characterized by a weight loss of about 3.1% upon heating to around 150° C., as measured by thermal gravimetric analysis.

In some embodiments, crystalline Type C of Formula IX is characterized by a differential scanning calorimetry thermogram comprising endothermic peaks at around 91.2 and 173.0° C.

In some embodiments, provided herein is crystalline Type G of Formula IX. In some embodiments, crystalline Type G of Formula IX is characterized by an X-ray powder diffraction pattern comprising peaks at 9.8, 12.2, 13.1, 13.4, and 14.6 degrees 2θ(±0.2 degrees 2θ). In some embodiments, crystalline Type G of Formula IX characterized by an X-ray powder diffraction pattern comprising peaks at 12.3, 13.2, 13.4, 17.8, and 26.6 degrees 2θ (±0.2 degrees 2θ). In some embodiments, crystalline Type G of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG. 43. In some embodiments crystalline Type G of Formula IX is substantially free of other crystalline forms.

In some embodiments, crystalline Type G of Formula IX is characterized by a weight loss a ranging from about 1.7% to about 2.7% upon heating to around 200° C., as measured by thermal gravimetric analysis. In some embodiments, crystalline Type G of Formula IX is characterized by a weight loss about 3.7% upon heating to around 200° C., as measured by thermal gravimetric analysis.

In some embodiments, crystalline Type G of Formula IX is characterized by a differential scanning calorimetry thermogram comprising endothermic peaks at around 231.1° C.

In some embodiments, provided herein is crystalline Type D of Formula IX. In some embodiments, crystalline Type D of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG. 45 (upper plot). In some embodiments crystalline Type D of Formula IX is substantially free of other crystalline forms.

In some embodiments, provided herein is crystalline Type F of Formula IX. In some embodiments, crystalline Type F of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG. 45 (lower plot). In some embodiments crystalline Type F of Formula IX is substantially free of other crystalline forms.

Methods of making the described crystalline types are further detailed in the Examples of this application. Crystallization conditions used for making types Types A-K include anti-solvent addition, slow evaporation, slow cooling, slurry conversion at room temperature (RT), slurry conversion at 50° C., solid vapor diffusion, liquid vapor diffusion.

IV. EXAMPLES Example 1 Preparation of 3-(3-Hydroxy-propyl)-pentane-2, 4-dione (a Compound of Formula IV).

A compound of Formula IIIa (100 g, 495 mmol 1.0 equiv.) was dissolved in acetone (1 L). A compound of Formula II (49.59 g, 495 mmol, 1.0 equiv.) was added to above solution, followed by addition of K₂CO₃ (82.14 g, 594.38 mmol, 1.2 equiv.) and KI (41.11 g, 247 mmol, 0.5 equiv.) at room temperature with stirring. The reaction mixture was heated to 60±5° C. and stirred for 40 h at this temperature. The reaction mixture was filtered and then concentrated under reduced pressure to afford a compound of Formula IV (102 g) as viscous orange liquid.

Example 2 Preparation of 3(3, 5-Dimethyl-1H-pyrazol-4-yl) propane-1-ol (a compound of Formula V).

A compound of Formula IV (100 g, 632 mmol, 1.0 equiv.) was dissolved in ethanol (1 L). Hydrazine hydrate (87 g, 1738 mmol, 2.75 equiv.) and conc. HCl (4.6 mL, 0.2 equiv.) were added to above solution at room temperature. The reaction mixture was heated to 75±5° C. and stirred for 3 h at this temperature. After completion of reaction by TLC (70% ethyl acetate: n-hexane, visible in iodine) and observation of product peak in mass spectrum, the reaction mixture was concentrated under reduce pressure to afford a compound of Formula V (70 g) as a colorless liquid syrup which was used as such for next step.

Example 3 Preparation of 4-(3-Bromo-propyl)-3, 5-dimethyl-1H-pyrazole (a compound of formula VIa).

A compound of Formula V (35 g, 227 mmol, 1.0 equiv.) was dissolved in 1, 2-dichloroethane (525 mL). PBr₃ (64.67mL, 681 mmol, 3 equiv.) was added in small portions at room temperature over 30 minutes. The reaction mixture was heated up to 75±5° C. and stirred for 3 h at this temperature. After completion of reaction by TLC (50% ethyl acetate: n-hexane, visible in iodine) and observation of product peak in Mass spectrum, the reaction mixture was diluted with dichloromethane (350mL) and quenched with saturated solution of NaHCO₃ till pH=7 to 8. Both organic and aqueous layers were separated and collected. The organic layer was dried over MgSO₄ and filtered. Filtrate was concentrated under reduce pressure to afford a compound of Formula VIa (38 g) as a viscous orange liquid.

Example 4 Preparation of 3-[3-(3, 5-Dimethyl-1H-pyrazol-4-yl)-propoxy]-4-fluoro-benzoic acid methyl ester (a compound of Formula VIIIa).

A compound of Formula VIIa (19 g, 111 mmol, 1.0 equiv.) was dissolved in DMF (190 mL). A compound of Formula VIa (31.5 g, 145.14 mmol, 1.3 equiv.) was added followed by K₂CO₃ (38.6 g, 279.18 mmol, 2.5 equiv.) at room temperature under stirred conditions. The reaction mixture was stirred for 16 to 18 h at room temperature. After completion of reaction in TLC (50% ethyl acetate: n- hexane), the reaction mixture was diluted with water (190 mL) and ethyl acetate (95 mL). Both organic layer and aqueous layer were separated and collected. Aqueous layer was extracted with ethyl acetate (190 mL). The combined organic extract was washed with water (95 mL), brine (95 mL), dried over Na₂SO₄ and filtered. The filtered organic layer was concentrated under reduce pressure to afford a crude viscous orange liquid (40 g). The crude was further purified by column chromatography using silica gel (285 g) and eluted with varying quantity of ethyl acetate in hexane to afford pure product, a compound of Formula VIIIa (25 g) as an off white solid.

Example 5 Preparation of 34343, 5-Dimethyl-1H-pyrazol-4-yl)-propoxy]-4-fluoro-benzoic acid methyl ester (a compound of Formula VIIIa).

4-(3-Bromopropyl)-3,5-dimethyl-1H-pyrazole hydrobromide (VIa) and DMSO were charged into vessel and agitated at 20±10° C. for 10 minutes. The mixture was then heated to 55±5° C. with stirring. To this mixture was transferred a stirred solution containing 4-fluoro-3-hydroxy-benzoic acid methyl ester (VIIa), potassium carbonate and anhydrous DMSO. The DMSO solution of the alkyl bromide were slowly transferred in order to maintaining an internal temperature of 55.0±5° C. Addition was complete after 6 hours and the mixture was agitated at 55.0±5° C. for an additional hour at 55.0±5° C. The mixture was cooled to 25±5° C. over the course of 30 minutes and water added while maintaining a temperature below 25° C. The mixture was extracted with ethyl acetate and the aqueous layer back extracted with ethyl acetate. The pooled ethyl acetate solutions were washed brine. The combined ethyl acetate washes were concentrated under vacuum to a minimal volume and heptane was added, which precipitates VIIIa. The mixture was heated to 75±5° C. and aged with stirring for 1 hour. The mixture was cooled to 25±5° C. over the course of two hours and the resulting solids collected by filtration. The filter cake was washed with ethyl acetate in heptane (30%). Isolated solids were dried with a nitrogen flow. Solids are charged to vessel and combined with ethyl acetate and heptane. The resulting mixture is heated to 75*5° C. to dissolve solids. The solution was cooled to 25±5° C. over the course of two hours and the resulting solids collected by filtration. The solids were washed with a 30% ethyl acetate/heptane solvent mixture and dried in vacuum oven at 55° C. to give VIIIa in >99.5% purity.

Example 6 Preparation of 3-[3-(3, 5-Dimethyl-1H-pyrazol-4-yl)-propoxy]-4-fluoro-benzoic acid (a compound of Formula IX)

A compound of Formula VIIIa (19 g, 62 mmol, 1 equiv.) was dissolved in methanol (95 mL, 5 vol.) at room temperature. A solution of LiOH.H₂O (6.5 g, 155 mmol, 2.5 equiv.) in water (57 mL) was added in small portions at room temperature over 10 to 15 minutes. The reaction mixture was stirred for 2 h at room temperature. After completion of reaction by TLC (70% ethyl acetate: n-hexane), the reaction mixture is concentrated below 45° C. under reduced pressure to afford a solid residue of Formula IX.

Example 7 Preparation of a Pharmaceutically Acceptable Salt of Formula I

The solid residue of Formula IX was dissolved in water (57 mL) and stirred for 10 min and cooled to 0±5° C. The aqueous solution was acidified with conc. HCl (20-25 mL) to pH=2 and stirred for 30 minutes at 0±5° C. Precipitation was observed which was filtered and dried at room temperature to afford pure product, a compound of Formula Ia (17.5 g) as an off-white solid.

Example 8 Additional Preparation of a Pharmaceutically Acceptable Salt of Formula I

Water and concentrated HCl were charged to a vessel and cooled with stirring to 10±5° C. Compound of Formula IX and water were charged to a second vessel and cooled with stirring to 10±5° C. The HCl solution in vessel 1 was transferred to a vessel containing compound of Formula IX mixture over not less than 15 minutes, while maintaining a temperature of <25° C. The resulting slurry was aged with stirring at 20±5° C. for 44 hours. The solids were collected by filtration, washed with 0.2 N HCl (3 ×) and dried under vacuum at >55° C. to provide Ia as white solid, >99.8% purity.

Example 9 Preparation of 3-[3-(3,5-dimethyl-1H-pyrazol-4-yl)propoxy]-4-fluorobenzoic acid hydrochloride salt (Compound Ia) from VIIIa

A jacketed glass vessel is charged with compound of formula VIIIa (1.0 equiv.) and methanol. The mixture is cooled with stirring to 10*5° C. and over the course of 20 minutes an aqueous solution of sodium hydroxide (3 equiv.) is charged. The mixture is aged with stirring at 20±5° C. for NLT 2 hours at which point the reaction is complete. Stirring is stopped and water is added. Methanol is then removed by vacuum distillation at an internal temperature of NMT 35° C. The resulting concentrated, clear aqueous solution is cooled to 10° C. and concentrated HCl is added until the pH was lowered to between 1.4-1.6 (pH meter) to precipitate the HCl salt. The solids are collected by filtration, washed with 0.2 N HCl and dried under vacuum at 50° C. to give a compound of Formula Ia in NLT 99.5% purity.

Example 10 Preparation of 3-[3-(3,5-dimethyl-1H-pyrazol-4-yl)propoxy]-4-fluorobenzoic acid (compound of formula IX) from VIIIa

Methyl 3-(3-(3,5-dimethyl-1H-pyrazol-4-yl)propoxy)-4-fluorobenzoate (Compound of formula VIIIa) and methanol were charged into a vessel and the resulting mixture was agitated at 20±5° C. until dissolved. The solution was cooled to 10±5° C. and over the course of 20 minutes a sodium hydroxide solution was added while maintaining a temperature ≤25° C. The mixture temperature was adjusted to 25±5° C. and aged with stirring for 18 hours. The reaction mixture was filtered . Water was added to filtrate and the resulting mixture concentrated under vacuum until volume of the mixture was reduced to minimal volume. Water was again added and the resulting mixture concentrated under vacuum until volume of the mixture was reduced to minimal volume. The pH of the aqueous mixture was adjusted to 5.5±0.5 by addition of concentrated hydrochloric acid then 0.5N HCl. The temperature of the mixture was adjusted to 7 ±5° C. and aged with stirring for an additional hour. The solids were collected by filtration, washed with water and partially dried under vacuum at ≥55° C. to provide compound of Formula IX as white solids with >99.5% HPLC purity.

Example 11 Conversion of the Hydrochloride Salt to Free Base

3-[3-(3, 5-Dimethyl-1H-pyrazol-4-yl)-propoxy]-4-fluorobenzoic acid hydrochloride (10.0 g, 30.4 mmol, 1.0 equiv.) was taken in deionized water (30.0 mL) at room temperature and was cooled to 10±5° C. To this mixture was added saturated sodium bicarbonate to pH≈6-7 and stirred for 30 minute at this temperature. The off white precipitate obtained was filtered and washed with deionized water (20 mL). Solid compound was dried at room temperature to afford 3-[3-(3,5-dimethyl-1H-pyrazol-4-yl)-propoxy]-4-fluorobenzoic acid (the compound of Formula IX) (7.40 g, 83.2%) as an off-white solid.

Example 12 Oral Dosing Using Pharmaceutical Acceptable Salt of Formula I

The following examples describes pharmacokinetic measurements of the compound of Formula IX in various salt and zwitterionic forms. The results shown herein show that compounds of Formula I possess an unexpectedly high pharmacokinetic profile.

Rats or Dogs were orally dosed with the zwitterion, Na salt, or HCl salt for of AG-10. The form of AG-10 used and dosing amount are as indicated in Table 3. Plasma samples from each rat/dog, as applicable, were measured between 0 and up to 96 hours after dosing with the specified form of AG-10. After isolation from the animal, each sample (50μL) was protein precipitated by adding 500 μL 0.1% formamide in acetonitrile to the sample. After addition for the formamide solution, the sample was vortexed and centrifuging at 1400 rpm for 15 min at 4° C. 100 μL of the supernatant was removed and diluted with 100 μL water. 5 μL of the diluted sample was injected for LC-MS/MS analysis. Pharmacokinetic data was calculated with the reported C_(max) and exposure (0-24h, ng.h/mL) shown in Table 3.

TABLE 3 Oral Dosing of a pharmaceutically acceptable salt of Formula I in multiple species Cmax Exposure Dose (ng/mL) (0-24 h) Species Form (mg/kg) (μM) ng · h/mL 1 Rat Zwitterion 200 26,373 (90.3 μM) — 2 Rat Na salt 50 mg/kg 12,322 (42.2 μM) — daily for 28 days 3 Rat HCl salt 200 216,333 (741 μM)  395,503 4 Dog Zwitterion 25 17,533 (60 μM)  89,786 5 Dog HCl salt 20 16,075 (55.1 μM) 69,088 6 Dog HCl salt 25 25,067 (85.8 μM) 128,374 7 Dog HCl salt 60  41,800 (143.2 μM) 155,814 8 Dog HCl salt 200 154,333 (528.5 μM) 1,020,640

As can be seen in Table 3, the HCl salt of Formula I provided a surprising and significant improvement in C_(max) values in dogs and rats as compared to the zwitterion and the Na salt. Compare, row 3 to row 1, and row 6 to row 4. Thus, in order to reach the same levels of bioavailability, a significantly smaller dose of the HCl salt of Formula I is needed.

Example 13 Intravenous Dosing Pharmaceutically Acceptable Salt of Formula I

The following examples describes pharmacokinetic measurements of the compound of Formula IX in various salt and zwitterionic forms when administered intravenously to Rats and Dogs. The results shown herein show that compounds of Formula I possess an unexpectedly high pharmacokinetic profile when administered both orally and intravenously.

Mice, Rats or Dogs were intravenously dosed with the zwitterion, Na salt, or HCl salt for of AG-10. The form of AG-10 and dosing amount are as indicated in Table 4. Plasma samples from each mouse/rat/dog, as applicable, were measured between 0 and 24 hours after dosing with the specified form of AG-10. After isolation from the animal, each sample (504) was protein precipitated by adding 500 μL 0.1% formamide in acetonitrile to the sample. After addition for the formamide solution, the sample was vortexed and centrifuging at 1400 rpm for 15 min at 4° C. 100 μL of the supernatant was removed and diluted with 100 μL water. 5 μL of the diluted samples was injected for LC-MS/MS analysis. Pharmacokinetic data was calculated with the reported C_(max) and exposure (0-24 h, ng.h/mL) shown in Table 4.

TABLE 4 Intravenous Dosing of a pharmaceutically acceptable salt of Formula I in multiple species Dose Cmax (ng/mL) Species Form (mg/kg) (μM) 1 Mouse Zwitterion 3 4,485 (15.4 μM) 2 Rat Zwitterion 3.43 3,093 (10.6 μM) 3 Rat Na salt 10 3,150 (10 μM) 4 Rat HCl salt 1 4,275 (14.6 μM)* 5 Dog HCl salt 1 5,959 (20.4 μM)* *the C_(max) reported is an extrapolated C₀

Example 14 High Bioavailability of AG-10 in Multiple Species

FIG. 2 shows pharmacokinetic results demonstrating the high bioavailability of AG-10 in dogs, rats, and mice. The mean plasma concentration of AG-10 was measured from 0-24 hours after dosing with 1 mg/kg of AG-10 intravenously and 5 mg/kg of AG-10 orally. The calculated pharmacokinetic data is shown in FIG. 2.

Example 15 High Bioavailability of AG-10 in Dogs

FIG. 3 shows pharmacokinetic results demonstrating the high bioavailability of AG-10 in male and female dogs at different dosing levels. The mean plasma concentration of AG-10 was measured from 2-24 hours after oral dosing with 5 mg/kg or 20 mg/kg of AG-10. The calculated pharmacokinetic data is shown in FIG. 3.

Example 16 Salt & Cocrystal Screen

Numerous salt and cocrystal conditions were tested targeting various pharmaceutically acceptable salts. Experimental details can be found in Table 5 and Table 6. Experiments were conducted using a variety of crystallization techniques including cooling, evaporation, slurrying, and solvent assisted grinding. Solids resulting from salt and cocrystal screening experiments were observed by polarized light microscopy (PLM) and analyzed by XRPD. XRPD patterns of isolated solids were compared to that of known forms of AG10 and counterion/coformer.

Confirmed salts of AG 10 were identified from experiments targeting salt formation with strong acids specifically methanesulfonic acid, ethane-1,2-disulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, ethanesulfonic acid, sulfuric acid, hydrogen bromide and nitric acid.

Salts or cocrystals of AG10 were also isolated from experiments targeting salt/cocrystal formation with weaker organic acids such as citric acid, acetic acid, maleic acid, oxalic acid and malic acid.

Experiments were also set up targeting L-tartaric acid, glycolic acid, and fumaric acid; however, experiments conducted aimed at isolating these materials as single crystalline phases were not successful and resulted in starting materials or a physical mixture of unique additional peaks with starting materials.

Attempts to generate salts/cocrystals of AG10 with L-aspartic acid, benzoic acid, gentisic acid, and succinic acid were not successful.

TABLE 5 Preparing Pharmaceutically Acceptable Salts of Formula I and Formula Ib Counterion (X:Y)^(a) Conditions^(b) Observations Results Benzenesulfonic 1. Added 2.0 mL THF to 94.7 mg 1. Hazy solution Fines, B/E acid AG-10 with stirring at 60° C. 2. Clear solution (Birefringence and (1:1) 2. Hot filtered 3. Clear solution extinction), 3. Added 52.3 mg acid to clear 4. Solution with solids agglomerates (PLM) solution, left at ET for ~15 min remain, solids collected Crystalline, AG10 4. Cooled to RT then stirred Besylate for ~3 d (XRPD) HBr 1. Added 2.0 mL heated MIBK to 1. Solids remain Fines, B/E, (1:1) 94.2 mg AG-10with stirring at 60° C. 2. Clear solution agglomerates (PLM) 2. Added 0.4 mL heated DMSO to 3. Yellow solution and oil Crystalline, AG10 sample with stirring at 60° C. formed Bromide 3. Added 55 μL HBr to sample 4. Oil with solids (XRPD) with stirring at 60° C. 5. Solution with solids 4. Cooled to RT, then placed in remain, solids collected VO at RT for 3 d 5. Added 1.0 mL MEK to sample with sonication and heated to 60° C., cooled × 2 Ethanesulfonic 1. Added 3.0 mL THF to 110.9 1. Cloudy solution Crystalline, AG10 acid mg AG-10at 50° C. with stirring 2. Clear solution Esylate (1:1) 2. Hot filtered 3. Solids precipitated (XRPD) 3. Added 31 μL acid to sample after ~1 min of with stirring at 50° C. stirring at ET 4. Cooled to RT 4. Solution with solids remain, solids collected 1,2-Ethanedisulfonic 1. Added 2.0 mL heated acetone 1. Solids remain Fines, acid to 101.8 mg AG-10with stirring 2. Clear solution agglomerates (1:1) at 50° C. 3. Solids precipitated (PLM) 2. Added 0.5 mL heated DMA to 4. Solution with solids Crystalline, AG10 sample with stirring remain, solids collected Edisylate + 3. Added 70 mg acid to sample ethanedisulfonic with stirring at 50° C. acid 4. Held at ET for ~10 min, (XRPD) cooled to RT 1,2-Ethanedisulfonic 1. 36.7 mg acid was dissolved in 1. Clear solution Fines, B/E, acid 400 μL acetone at 60° C. 2. Clear solution agglomerates (PLM) (2:1) 2. 106.5 mg AG-10was dissolved 3. Hazy solution Crystalline, AG10 in 2.0 mL DMA at 60° C. 4. Solution with solids Edisylate 3. Acid solution was added to remain, solids collected (XRPD) AG-10 solution at ET with stirring and then cooled to RT 4. Heated to 60° C., then cooled Methanesulfonic 1. Added 2.0 mL heated MEK to 1. Solids remain Fines, acid 100.7 mg AG-10 with stirring at 2. Clear solution agglomerates (1:1) 50° C. 3. Solids precipitated (PLM) 2. Added 0.3 mL heated DMF to 4. Solution with solids Crystalline, AG10 sample with stirring remain, solids collected Mesylate 3. Added 23 μL acid to sample (XRPD) with stirring at 50° C. 4. Held at ET for ~20 min, cooled p-toluenesulfonic 1. Added 3.5 mL ACN to112.0 1. Solids present Fines, acid mg AG-10 with stirring at 60° C. 2. Solution cleared agglomerates (1:1) 2. Added 74.5 mg acid to slurry at ET briefly, then precipitation (PLM) 3. Increased to 70° C. with stirring 3. Clear solution with Crystalline, AG10 4. Mixed solids back into solids on sides Tosylate solution, cooled to RT 4. Solution with solids (XRPD) remain, solids collected Nitric acid 1. Added 0.7 mL DMSO to 95.8 1. Clear solution Crystalline, AG10 (1:1) mg AG-10 with stirring at RT 2. Clear solution Nitrate Form A 2. Added 330 μL acid to solution 3. Solution with solids (XRPD) at RT remain, solids collected 3. Left at RT stirring for 2 d Nitric acid 1. Added 3.0 mL THF to 97.2 mg 1. Solids remain Crystalline, AG10 (1:1) AG-10 with stirring at RT 2. Clear solution Nitrate Form B 2. Added 330 μL acid to solution 3. Clear solution (XRPD) at RT 4. Solids remain 3. Left at RT stirring for 2 d 4. Placed in VO at RT Sulfuric 1. Added 2.0 mL heated EtOH to 1. Opaque solution Fan agglomerates, acid 108.5 mg AG-10 with stirring at 2. Clear solution B/E (PLM) (1:1) 60° C. 3. Cloudy solution Crystalline, AG10 2. Hot filtered 4. Hazy solution Sulfate (XRPD) 3. Added 22 μL acid to sample 5. Hazy solution remains with sitting at 60° C. 6. Clear solution 4. Cooled to RT and stirred for 2 d 7. Solids remain 5. Placed at 2-8° C. for 5 d 6. Evaporation at RT for 11 d 7. Evaporation under nitrogen for 2 d ^(a)X:Y = AG-10:Counterion mole ratio ^(b)Temperatures and times are approximate

TABLE 6 Preparing Pharmaceutically Acceptable Salts of Formula I and Formula Ib Coformer (X:Y)^(a) Conditions^(b) Observations Results Acetic acid 1. Added 2.0 mL heated EtOH to 1. Clear solution Fines, B/E, (1:2.2) 85.0 mg AG-10 with stirring at 70° C. 2. Solids present agglomerates (PLM) 2. Added 37 μL acetic acid to 3. Clear solution Crystalline AG10 sample with stirring at 70° C. 4. Solution with solids (Type G [2]) 3. Cooled to RT, held overnight, and remain, solids collected (XRPD) reheated to 60° C. for ~3 h 4. Cooled to RT and stirred for ~2 d Acetic acid 1. Milled 102.7 mg AG-10 with 40 1. Solids turned into a Crystalline, AG10 (1:1) μL acetic acid paste then dried and Acetic acid Form A white solids collected (XRPD) Citric acid 1. Added 3.0 mL saturated citric 1. Solids present AG10 citrate (excess) acid solution in IPA to 78.3 mg AG- 2. Clear solution (SCXRD) 10 with stirring at RT 3. Clear solution Citric acid + 2. Stirred at RT at 1 d 4. Single crystal collected AG10 citrate 3. Placed at 2-8° C. for 1 d 5. Solids with liquid remain (XRPD) 4. Evaporated at RT after 10 d 6. Solids remain used 5. Returned to evaporate at RT for for XRPD 7 d and then placed in VO for ~1 d 6. Placed to evaporate under nitrogen for 1 d Citric acid 1. Added 1.6 mL IPA to 72.9 mg 1. Clear solution Fines, (1:1) acid at 50° C. 2. Solids remain agglomerates, 2. Added acid solution to 110.1 mg 3. Solution with solids B/E (PLM) AG-10 with stirring at 50° C. remain, solids collected Crystalline AG10 overnight (XRPD) 3. Cooled to RT with stirring, left overnight L-Malic acid 1. Added 6.0 mL anhy. ACN to 81.1 1. Solids remain Starburst, (1:1) mg AG-10 with stirring at 50° C. 2. Cloudy solution agglomerates, 2. Added 2.0 mL DCM to sample 3. Clear solution B/E with gel with stirring at 50° C. 4. Clear solution (PLM) 3. Hot filtered 5. Clear solution 4. Added acid to solution with 6. Solution remains stirring at 50° C. 7. Solids with gel remain 5. Cooled to RT and left stirring at RT 6. Evaporation at RT 7. Placed in VO at RT L-Malic acid 1. Added 1.0 mL saturated L-malic 1. Clear solution Fines, B/E, (excess) acid solution in ACN to 89.3 mg 2. Clear solution agglomerates (PLM) AG-10 with stirring at 60° C. 3. Thick slurry, solids Crystalline, AG10 2. Cooled to RT and stirred for ~3 d collected L-malic acid 3. Placed at 2-8° C. for 3 d (XRPD) Maleic acid 1. Dissolved 85.7 mg maleic acid in 1. Clear solution Fines, B/E, (1:2.2) 1.5 mL Nitromethane with stirring 2. Solids remain agglomerates (PLM) at 70° C. 3. Solids remain AG10 Maleate 2. Added acid solution to 87.8 mg 4. Solution with solids Form B + AG-10 with stirring at ET remain, chalky solids Material A 3. Cooled to RT, held overnight, and collected (XRPD) reheated to 60° C. for ~3 h 4. Cooled to RT and stirred for ~2 d Maleic acid 1. LIMS 471312 dried at 110° C. 1. Solids became gel-like Agglomerates with for ~7 min with yellow tint minor B/E 2. Cooled to RT 2. Solids remain (PLM) Disordered AG10 Maleate Form B + amorphous halo (XRPD) Maleic acid 1. 2.0 mL p-dioxane was added to 1. Solids remain AG10 Maleate Form B (1:1) 49.6 mg acid was and 122.9 mg AG- 2. Solids remain (XRPD) 10 at RT with stirring 3. Solution with solids 2. Slurry was heated to 50° C. and remain, solids collected 4.0 mL p-dioxane was added at 50° C. with stirring. 3. Stirred at 50° C. for ~2 d Maleic acid 1. Filtrate of LIMS 474152 1. Solids remain AG10 Maleate Form B + (1:1) suspension was left to evaporate additional peak 16.4° + at RT amorphous halo (XRPD) Oxalic acid 1. Added 4.0 mL anhy Acetone and 1. Cloudy solution Crystalline AG10 (1:1.1) 1.0 mL DMA to 96.2 mg AG-10 2. Clear solution Oxalate with stirring at 50° C. 3. Clear solution, then (XRPD) 2. Hot filtered precipitation of solids 3. 34.5 mg acid added to sample at 4. Solution with solids 50° C. remain, solids collected 4. Cooled to RT ^(a)X:Y = AG-10:Counterion mole ratio ^(b)Temperatures and times are approximate

Example 17 The Preparation of the Mesylate Salt of Formula IX

The mesylate salt of Formula DC was produced upon the addition of 1 molar equivalent of methanesulfonic acid to an AG-10 MEK: DMF 2:0.3 v/v solution at elevated temperature. The suspension was held at elevated temperature for ˜20 minutes, cooled to room temperature and solids isolated.

The XRPD pattern is shown in FIG. 4 and exhibits resolution of peaks indicative of crystalline material. Indexing of the XRPD pattern was attempted; however, an indexing solution was not found, possibly due to the sample containing a mixture of crystalline phases or low peak resolution.

The ¹H NMR spectrum was consistent with AG-10 mesylate salt in a 1:1 mole ratio based on the peak at 2.37 ppm. Trace amounts of DMF and additional unknown peaks were also observed in the spectrum.

The DSC thermogram (FIG. 5) shows a single endotherm at approx. 233° C. (peak max) likely attributable to melting. No significant weight loss is observed in the TGA (FIG. 5) up to ˜200° C. suggesting the material is likely unsolvated/anhydrous.

Example 18 The Preparation of the Edisylate Salt of Formula IX

The edisylate salt of Formula IX was produced upon the addition of 1 molar equivalent of 1,2-ethanedisulfonic acid to an AG10 acetone: DMA solution at elevated temperature. The suspension was cooled to ambient temperature and solids isolated.

By XRPD, the edisylate salt of Formula IX is composed of a crystalline material (FIG. 6). The ¹H NMR spectrum is consistent with AG-10 edisylate in a 2:1 mole ratio based on the peak at 2.7 ppm. Approximately one mole of DMA was also observed suggesting an AG-10 edisylate DMA (2:1:1) solvate.

The DSC thermogram (FIG. 7) shows a broad feature at ˜139° C. associated with a weight loss of 11% based on TGA (FIG. 7) data that is likely due to desolvation. A sharper endotherm at 313° C. (peak max) likely attributable to melt/decomposition of the desolvated material is observed. Hot stage microscopy is suggested to further understand the behavior of the material with heating.

A sample of the edisylate salt of Formula IX was dried at 180° C. for 10 minutes and no change in physical form resulted based on XRPD.

Example 19 The Preparation of the Besylate Salt of Formula IX

The besylate salt of Formula IX was prepared from cooling a THF solution containing equimolar equivalents of AG-10 and benzenesulfonic acid.

The besylate salt of Formula IX is composed of a crystalline material and the XRPD pattern in shown in FIG. 8. The ¹H NMR spectrum is generally consistent with AG10 besylate salt in an approximate 1:1 ratio. Trace amounts of THF was also observed in the spectrum based on the peak at 3.6 ppm.

Two endothermy are observed in the DSC thermogram with peak max at ˜158° C. and 177° C. (FIG. 9). A weight loss of 0.2% is observed between 42° C. and 127° C. (FIG. 9).

Example 20 The Preparation of the Tosylate Salt of Formula IX

The tosylate salt of Formula IX was produced upon the addition of 1 molar equivalent of p-toluenesulfonic acid to an AG-10 acetonitrile solution at elevated temperature.

By XRPD, the tosylate salt of Formula IX is composed of a crystalline material (FIG. 10). The pattern was successfully indexed indicating it is composed primarily or exclusively of a single crystalline phase. The unit cell volume obtained from the indexing solution is consistent with AG10 tosylate 1:1 salt based on considerations of molecular volume.

The ¹H NMR spectrum is overall consistent with an AG10 tosylate salt in an approximate 1:1 mole ratio based on the peak at 2.28 ppm.

The DSC thermogram shows a single endotherm at approx. 205° C. (peak max) likely attributable to melting (FIG. 11). No significant weight loss is observed in the TGA up to ˜160° C. suggesting the material is likely unsolvated/anhydrous (FIG. 11).

Example 21 The Preparation of the Esylate Salt of Formula IX

The esylate salt of Formula IX precipitated from a THF solution containing AG-10 and ethanesulfonic acid (1:1 mole ratio) at 50° C. The suspension was cooled and solids isolated.

The esylate salt of Formula IX is composed of a crystalline materials as confirmed by XRPD (FIG. 12). The ¹H NMR spectrum is consistent with an AG10 esylate salt in a 1:1 mole ratio based on the peak at 2.4 ppm. THF, approximately 0.1 mole was observed in the spectrum.

A single endotherm at 199° C. (peak max) is observed in the DSC thermogram likely due to melting (FIG. 13). No significant weight loss upon heating up to the melt which may suggest the material is unsolvated or anhydrous (FIG. 13).

Example 22 The Preparation of the Bromide Salt of Formula IX

The bromide salt of Formula IX was prepared via the addition of an equimolar amount of hydrogen bromide to a MIBK: DMSO 2:0.4 v/v solution of AG-10 at ˜60° C. This produced a yellow solution and oil formed. The sample was placed in a vacuum oven at room temperature for 3 days and resulted in oil with solids present. MEK was added to the sample with sonication, and heated to 60° C. then cooled, twice. Solids remaining in the resulting suspension were isolated and analyzed.

The bromide salt of Formula IX is composed of a crystalline material (FIG. 14). The ¹H NMR spectrum is consistent with the chemical structure of AG-10. DMSO, approximately 1 mole was also obscrvcd based on the peak at 2.54 ppm.

The bromide content was found to be 17.7% by mass based on IC and is in agreement with the calculated bromide content (17.7%) of an AG10 bromide DMSO 1:1:1 solvate.

An endotherm at ˜105° C. (peak max), followed by an exotherm at 155° C. (peak max) and an endotherm at ˜214° C. is observed in the DSC data (FIG. 15). A weight loss of 19.9% is observed upon heating up to ˜182° C., likely associated with the loss of solvent and possible recrystallization into an unsolvated form (FIG. 15).

Example 23 The Preparation of the Nitrate Salt of Formula IX

Two nitrate salt forms of Formula IX were identified. The two forms are referred to as Form a and Form b.

Nitrate Salt, Form a

Form a of the nitrate salt of Formula IX precipitated from a DMSO solution containing AG10 and nitric acid in equimolar ratios. AG-10 Nitrate Material A is a composed of a single crystalline phase based on successful indexing of the XRPD pattern (FIG. 16).

The solution ¹H NMR spectrum for AG10 Nitrate Form A is consistent with the chemical structure of AG-10. DMSO, approximately 0.8 mole is present based on the peak at 2.54 ppm. Water and minor additional peaks are also observed.

The DSC thermogram exhibits a broad endotherm at ˜117° C., which is associated with a weight loss of 2.5% likely attributable to the loss of volatiles (FIG. 17). The broad endotherm is followed be an exotherm with peak maximum at ˜173° C., that is associated with a weight loss of ˜16% likely due to melt/decomposition (FIG. 17).

The nitrate content was found to be 7.5% by mass based on IC which is not consistent with the calculated nitrate content anticipated for a unsolvated 1:1 nitrate salt (theoretical nitrate content: 17.5%) or even a 1:1:1 AG10 nitrate DMSO solvate (theoretical nitrate: 14.3%).

Nitrate Salt, Form b

Form b of the nitrate salt of Formula IX was prepared by evaporation of a THF solution containing AG-10 and nitric acid in an equimolar ratio. The XRPD pattern of this solid is shown in FIG. 18.

The solution ¹H NMR spectrum for AG10 Nitrate Material B is consistent with the chemical structure of AG-10.

The nitrate content was found to be 16.9% by mass based on IC, and is in general agreement with an approximately 1:1 AG-10 nitrate salt.

Example 24 The Preparation of the Sulfate Salt of Formula IX

The sulfate salt of Formula IX was prepared by evaporation of an ethanol solution containing equimolar amounts of AG-10 and sulfuric acid that was produced upon cooling (60° C. to 2-8° C.). The sulfate salt of Formula IX is composed of a crystalline material (FIG. 19).

The¹H NMR spectrum confirms the presence of AG 10 and contains approximately 1 mole of ethanol based on the peaks at 1.06 and 3.4 ppm. Additional unknown peaks were also observed in the spectrum.

The sulfate content was found to be 15.9% by mass based on IC, which corresponds to an AG10: sulfate ratio of 1:0.58.

A weight loss of 6.4% is observed in the TGA thermogram between ˜30° C. and 96° C., equivalent to 1 mole ethanol, assuming an AG10 sulfate 2:1 salt (FIG. 20). Broad features are observed by DSC (FIG. 20).

Example 25 The Preparation of the Citrate Salt of Formula IX

Single crystals of the citrate salt of Formula IX were obtained after an IPA solution saturated with citric acid and containing AG-10 was allowed to evaporate at room temperature. After recovery of a suitable single crystal for SCXRD, the sample was allowed to evaporate further and solids collected were composed of a mixture of AG-10 citrate and citric acid based on XRPD.

The structure of AG10 citrate was determined successfully. The crystal system is triclinic and the space group is PT. The cell parameters and calculated volume are: a=7.64605(12) Å, b=8.37443(13) Å, c=17.8097(3) Å, α=87.9509(14)°, β=79.7770(14)°, γ=88.3139(13)°, V =1121.24(3) Å³. The formula weight is 484.43 g mol⁻¹ with Z=2, resulting in a calculated density of 1.435 g cm⁻³.

A second experiment was performed aimed at obtaining bulk solids of AG 10 citrate as a single crystalline phase for further characterization. The experiment also resulted in a physical mixture of AG10 citrate and citric acid.

Example 26 The Preparation of the Oxalate Salt of Formula IX

The oxalate salt of Formula IX precipitated from a DMA solution containing AG10 and oxalic acid (1:1 mole ratio) at 50° C. The sample was cooled to room temperature and solids isolated for characterization.

By XRPD, the oxalate salt of Formula IX is composed of a crystalline material (FIG. 21). The XRPD pattern of the sample was successfully indexed indicating the sample is composed primarily or exclusively of a single crystalline phase. The indexed volume is consistent with an AG10 hemi-oxalate based on considerations of molecular volume.

¹H NMR spectrum is consistent with the chemical structure of AG-10. DMA, approximately 0.1 mole and water were also present in the spectrum.

By IC, the oxalate content of the sample was determined to be 13.7%, confirming the ˜2:1 stoichiometry of AG 10 hemi-oxalate salt.

A single endotherm at ˜225° C. (peak max) is observed in the DSC data likely attributable to melt/decomposition based on TGA data (FIG. 22). The TGA thermogram exhibits an initial weight loss of 0.9% upon heating between 33° C. and 169° C., likely due to the loss of residual surface solvent such as DMA which was observed by ¹H NMR (FIG. 22).

Example 27 The Preparation of the Maleate Salt of Formula IX

Two maleate salt forms of Formula IX were identified. The two forms are referred to as Form a and Form b.

Maleate Salt, Form a

The addition of a nitromethane solution of maleic acid (2.2 mole equivalents) to AG-10 at 70° C. resulted in a suspension. The suspension was cooled to room temperature and reheated to 60° C. twice before solids were isolated.

Form a of the maleate salt of Formula IX is composed of a crystalline material based on

XRPD (FIG. 23). The XRPD pattern was not able to be indexed which suggest the material is not composed of a single crystalline phase and is a possible mixture of forms. XRPD analysis suggests that form a of the maleate salt of Formula IX was isolated as a mixture of form b of the maleate salt of Formula IX.

The ¹H NMR spectrum of the sample contained AG-10: maleic acid in approximate 1:1 mole ratio based on the peak at 6.23 ppm. Approximately 1.3 moles nitromethane is observed for each mole of AG10 based on the presence of the peak at 4.42 ppm. Minor additional unknown peaks were also observed in the spectrum.

An endotherm at ˜160° C. (peak max) is observed in the DSC data (FIG. 24). A weight loss of 8.4% is seen upon heating to 110° C. which may be due to the loss of solvent (FIG. 24). A sample of form a of the maleate salt of Formula IX was dried at 110° C. for approximately 7 minutes and resulted in disordered material with peaks consistent with form b of the maleate salt of Formula IX based on XRPD.

The sample is likely composed of a mixture of form b of the maelate salt of Formula IX and a possible nitromethane solvate based on XRPD and ¹H NMR data.

Maleate Salt, Form b

Form b of the maleate salt of Formula IX was produced from an elevated temperature slurry experiment containing AG-10 and maleic acid (1:1) in p-dioxane. The XRPD pattern of form b of the maleate salt of Formula IX (FIG. 25) was successfully indexed indicating the pattern is composed primarily or exclusively of a single crystalline phase. The indexed volume is consistent with a 1:1 AG10 maleate salt.

The ¹H NMR spectrum of the sample is consistent with AG10 and maleic acid in a 1:1 mole ratio. Approximately 0.3 moles p-dioxane was also observed in the spectrum.

A single endotherm is observed at ˜171° C. (peak max) in the DSC thermogram (FIG. 26). No significant weight loss is observed upon heating the sample between 33° C. and 120° C. (FIG. 26).

Example 28 The Preparation of the Acetic Acid Salt of Formula IX

The acetic acid salt of Formula IX was produced by directly milling AG-10 with acetic acid in a 1:1 mole ratio.

By XRPD, the acetic acid salt of Formula IX is composed of a crystalline material and is shown in FIG. 27. The XRPD pattern was successfully indexed indicating the sample is composed primarily or exclusively of a single crystalline phase.

The ¹H NMR spectrum is consistent with the chemical structure of AG-10, with approximately 0.9 mole acetic acid present.

The DSC thermogram showed a broad endotherm at ˜113° C. that is associated with a weight loss of ˜16% likely due to the loss of acetic acid (FIG. 28). This is followed by endotherms at 186° C. and 192° (peak max) that are likely attributable to melting of AG10 free form (FIG. 28).

Example 29 The Preparation of the L-Malic Acid Salt of Formula IX

Cooling of a solution produced by the addition of a saturated solution of L-malic acid in nitromethane to AG-10 at 60° C., produced solids at sub-ambient temperature. The XRPD pattern is composed of a unique crystalline material designated the L-malic acid salt of Formula IX (FIG. 29).

¹H NMR spectrum contained 1.8 moles of malic acid per mole of AG-10 based on the peak at 4.2 ppm. Minor ACN and water were also observed in the spectrum.

Two broad endotherms are observed in the DSC data with peak maxima at ˜89° C. and 199° C. (FIG. 30). A weight loss of 0.2% is observed between 33° C. and 107° C. (FIG. 30).

Example 30 Crystalline Type A of Formula IX

The material of Formula Ia (HCl salt of Formula IX) as prepared in Example 7 was characterized by X-ray powder diffraction (XRPD) (FIG. 31), thermo-gravimetric analysis (TGA) (FIG. 32-FIG. 34), differential scanning calorimetry (DSC) (FIG. 32-FIG. 34) and polarized light microscopy (PLM) (FIG. 35). This material was referred to as crystalline Type A of Formula IX. Three different XRPD plots are overlaid in FIG. 31 showing three different preparations according to Example 7.

Representative peak values of the XRPD plots shown in FIG. 31 are provided in Table 7, below.

TABLE 7 Representative XRPD peak values for Crystalline Type A of Formula IX Position [°2θ] Relative Intensity [%] 7.0 23.50 10.4 21.74 12.0 51.77 13.0 6.45 13.9 34.17 15.6 31.64 17.0 31.28 18.6 15.02 20.8 19.23 21.8 100.00 23.3 12.57 24.2 14.33 24.7 14.29 25.3 17.29 25.9 63.60 26.7 55.94 27.9 44.84 28.2 12.96 28.6 10.35 29.1 13.20 30.9 13.10 31.6 10.76 33.8 2.95 34.5 4.62 37.9 4.55

Three separate TGA/DSC plots of crystalline Type A of Formula IX are shown in FIG. 32 to FIG. 35. Weight losses of about 0.7% to 1.9% upon heating to around 150° C. was measured by thermal gravimetric analysis, and further characterization using differential scanning calorimetry shows at least two endothermic peaks at about 211-214° C. and 237-239° C. The HPLC purity of crystalline Type A of Formula IX was determined to be 98.76 area %.

The asymmetric unit of crystalline Type A of Formula IX is shown in FIG. 36. It includes one cation of compound AG 10 freebase and one chloride ion (the HCl molecule transferred the proton to N1 atom of freebase), indicating Type A was an anhydrous mono-HCl salt form.

To evaluate the hygroscopicity and physical stability of crystalline Type A of Formula IX under different humidity, dynamic vapor sorption (DVS) data was collected at 25° C. after the sample was pre-equilibrated at 0% RH to remove unbounded water. DVS result (FIG. 37) showed a water uptake of 1.6% at 25° C/80% RH, suggesting crystalline Type A of Formula IX slightly hygroscopic. Additionally, XRPD results (FIG. 38) showed no form change before and after DVS test.

Example 31 Polymorphic Screen of AG-10

Using crystalline Type A of Formula IX as the starting material, polymorph screening experiments were performed under 98 conditions, through methods of vapor diffusion, anti-solvent addition, slurry conversion, slow evaporation, and slow cooling. From polymorph screening and follow-up investigation, a total of ten additional crystal forms were obtained including six HCl salt forms (Type A/B/E/H/I/J), two freebase forms (Type C/G) and two currently unidentified forms (Type D/F). Forms of Type A/B/E were identified to be anhydrates. Type I was identified to be a hydrate. Type H and J were identified to be a MeOH solvate and DMAc solvate, respectively. The methods utilized and crystal forms identified are summarized in Table 8.

TABLE 8 Summary of polymorph screening of AG 10 No. of Method Experiments Isolated Solid Forms Anti-solvent addition 24 Type A, C, D, E, Type A + extra peaks Slow evaporation 7 Type A, H Slow cooling 8 Type A, E, J Slurry conversion 25 Type A, C, F, G Solid vapor diffusion 13 Type A, Type A + extra peaks Liquid vapor diffusion 21 Type A Total 98 Type A, C, D, E, F, G, H, J, Type A + extra peaks

Anti-Solvent Addition

A total of 24 anti-solvent addition experiments were carried out. For each experiment, about 15 mg of crystalline form Type A of Formula IX was weighed into a 20-mL glass vial, followed by the addition of 0.125-0.63 mL corresponding solvent. The mixture was then magnetically stirred at the speed of 750 RPM to get a clear solution at RT. Subsequently, the corresponding anti-solvent was added to the solution to induce precipitation or until the total amount of anti-solvent reached 10.0 mL. The clear solutions were transferred to slurry at 5° C. If no precipitation occurred, the solution was then transferred to fast evaporation at RT or vacuum drying at RT. The solids were isolated for XRPD analysis. Results summarized in Table 9 showed that Type A, C, D, E and Type A with extra peaks were obtained.

TABLE 9 Summary of anti-solvent addition experiments Solvent Anti-Solvent Final Results DMSO IPA Type A + one peak** Acetone Type A + one peak* EtOAc Type A THF Type A** ACN Type A* Toluene Type E DCM Type A H₂O Type C MeOH MEK Type A IPAc Type D 2-MeTHF Type A* CPME Type A ACN Type A H₂O Type C CHCl₃ Type A + extra peaks** Toluene Type A + extra peaks EtOH MIBK Type A EtOAc Type A** 1,4-Dioxane Gel Anisole Type A** ACN Type E* DCM Type A** n-Heptane Type A H₂O Type C *solids were obtained via stirring at 5° C. **solids were obtained via fast evaporation or vacuum drying at RT.

Slow Evaporation

Slow evaporation experiments were performed under 7 conditions. For each experiment, around 15 mg of crystalline Type A of Formula IX was weighed into a 3-mL glass vial, followed by the addition of corresponding solvent or solvent mixture to get a clear solution. Subsequently, the vial was covered with parafilm with 3-4 pinholes, and kept at RT to allow the solution to evaporate slowly. The isolated solids were tested by XRPD. As summarized in Table 10, Type A and H were generated.

TABLE 10 Summary of slow evaporation experiments Solvent (v:v) Final Results MeOH Type A EtOH Type A DCM/MeOH, 1:1 Type A Acetone/MeOH, 4:1 Type H EtOAc/EtOH, 4:1 Gel THF/MeOH, 4:1 Type A ACN/EtOH, 4:1 Type A

Slow Cooling

Slow cooling experiments were conducted in 8 solvent systems. For each experiment, about 15-35 mg of crystalline Type A of Formula IX was suspended in 0.8-2.0 mL of corresponding solvent in a 3-mL glass vial at RT. The suspension was transferred to slurry at 50° C. with a magnetic stirrer at the speed of 750 RPM. The sample was equilibrated at 50° C. for 1 hr. and filtered using a 0.45 μm PTFE membrane. Subsequently, the filtrate was slowly cooled down from 50° C. to 5° C. at a rate of 0.1° C./min. If no precipitation occurred, the solution was then transferred to fast evaporation at RT or vacuum drying at RT. The results summarized in Table 11 indicated that Type A, E and J were obtained.

TABLE 11 Summary of slow cooling experiments Solvent, v:v Final Results* EtOH Type E IPA Type A** MEK/DMAc, 3:1 Type J** IPAc/EtOH, 3:1 Type A Anisole/MeOH, 3:1 Type A ACN/NMP, 3:1 Clear solution H2O/DMAc, 3:1 Type J** CHCl₃/EtOH, 3:1 Type E *all the samples were transferred to slow evaporation at RT. **solids were obtained after vacuum drying at 50° C. was performed on the clear solutions.

Slurry Conversion at RT

Slurry conversion experiments were conducted at RT in different solvent systems. For each experiment, about 15-35 mg of crystalline Type A of Formula IX was suspended in 0.3-2.0 mL corresponding solvent in a 1.5-mL glass vial. After the suspension was magnetically stirred for 4 days at RT, the remaining solids were isolated for XRPD analysis. Results summarized in Table 12 showed that Type A, C and G were obtained.

TABLE 12 Summary of slurry conversion experiments at RT Solvent, v:v Final Results EtOH Type A H₂O Type C EtOH/H₂O, 97:3, a_(w) = 0.2 Type A EtOH/H₂O, 927:73, a_(w) = 0.4 Type A EtOH/H₂O, 86:14, a_(w) = 0.6 Type A EtOH/H₂O, 71:29, a_(w) = 0.8 Type A H₂O/DMAc, 3:1 Type G MIBK/MeOH, 1:1 Type A THF/H₂O, 9:1 Type A ACN/EtOH, 3:1 Type A DCM/DMSO, 3:1 Type A EtOAc/DMF, 3:1 Type A

Slurry Conversion at 50° C.

Slurry conversion experiments were conducted at 50° C. in different solvent systems. For each experiment, about 15 mg of crystalline Type A of Formula IX was suspended in 1.0 mL corresponding solvent in a 1.5-mL glass vial. After the suspension was magnetically stirred for 4 days at 50° C., the remaining solids were isolated for XRPD analysis. Results summarized in Table 13 indicated that Type A and F were obtained.

TABLE 13 Summary of slurry conversion experiments at 50° C. Solvent Temperature, ° C. Final Results IPA 50 Type A CHCl₃ Type A Acetone Type A MEK Type A IPAc Type A EtOAc Type A Anisole Type A THF Type A 2-MeTHF Type A 1,4-Dioxane Type A ACN Type A Toluene Type F 1-Butanol Type A

Solid Vapor Diffusion

Solid vapor diffusion experiments were conducted using 13 solvents. For each experiment, about 15 mg of crystalline Type A of Formula IX was weighed into a 3-mL vial, which was placed into a 20-mL vial with 4 mL of corresponding solvent. The 20-mL vial was sealed with a cap and kept at RT for 39 days to allow the solvent vapor to interact with the solid sample. The isolated solids were tested by XRPD. The results summarized in Table 14 indicated that Type A and Type A with extra peaks were obtained.

TABLE 14 Summary of solid vapor diffusion experiments Solvent Final Results H₂O Type A DCM Type A EtOH Type A MeOH Type A ACN Type A THF Type A CHCl₃ Type A Acetone Type A DMF Type A EtOAc Type A 1,4-Dioxane Type A IPA Type A DMSO Type A + extra peaks

Liquid Vapor Diffusion

Twenty-one liquid vapor diffusion experiments were conducted. For each experiment, about 15 mg of crystalline Type A of Formula IX was dissolved in 0.125-0.6 mL of corresponding solvent to obtain a clear solution in a 3-mL vial. Subsequently, the solution was placed into a 20-mL vial with 4 mL of corresponding anti-solvent. The 20-mL vial was sealed with a cap and kept at RT, allowing sufficient time for solvent vapor to interact with the solution. If no precipitation occurred, the solution was then transferred to fast evaporation at RT. Solids were isolated for XRPD analysis. Results summarized in Table 15: showed that Type A was obtained.

TABLE 15 Summary of liquid vapor diffusion experiments Solvent, v:v Anti-solvent Final Results DCM/MeOH, 1:1 Toluene Type A MIBK Type A EtOAc Type A ACN Type A Anisole Type A EtOH MEK Type A IPAc Type A 2-MeTHF Type A ACN Type A H₂O Type A CHCl₃ Type A n-Heptane Type A Toluene Type A DMF IPA Clear solution Acetone Clear solution EtOAc Clear solution THF Clear solution ACN Clear solution Toluene Clear solution H₂O Limited solid DCM Clear solution

A chart summarizing the interconversions between the identified crystal forms is shown in FIG. 39.

Abbreviation for Solvents

TABLE 16 Solvent abbreviation list Abbreviation Solvent Abbreviation Solvent MeOH Methanol THF Tetrahydrofuran EtOH Ethanol 2-MeTHF 2-Methyltetrahydrofuran IPA Isopropyl alcohol ACN Acetonitrile MIBK 4-Methyl-2-pentanone DMSO Dimethylsulfoxide EtOAc Ethyl acetate DCM Dichloromethane IPAc Isopropyl acetate DMAc N,N-Dimethylformamide MTBE Methyl tert-butyl ether MEK Methylethyl ketone

Instruments and Methods XRPD

For XRPD analysis, PANalytical X-ray powder diffractometers were used. The XRPD parameters used are listed in Table 17.

TABLE 17 Parameters for XRPD test PANalytical PANalytical Parameters (Reflection Mode) (Reflection Mode) Model Empyrean X′ Pert³ X-Ray wavelength Cu, kα, Cu, kα, Kα1 (Å): 1.540598, Kα1 (Å): 1.540598, Kα2 (Å): 1.544426 Kα2 (Å): 1.544426 Kα2/Kα1 intensity Kα2/Kα1 intensity ratio: 0.50 ratio: 0.50 X-Ray tube setting 45 kV, 40 mA 45 kV, 40 mA Divergence slit Automatic ⅛° Scan mode Continuous Continuous Scan range (2TH) 3°-40° 3°-40° Scan step time (s) 17.8 46.7 Step size (°2TH) 0.0167 0.0263 Test Time 5 min 30 s 5 min 04 s

TGA & DSC

TGA data was collected using a TA Q500/Q5000 TGA from TA Instruments. DSC was performed using a TA Q200/Q2000 DSC from TA Instruments. Detailed parameters used are listed in Table 18.

TABLE 18 Parameters for TGA and DSC test Parameters TGA DSC Method Ramp Ramp Sample pan Platinum, open Aluminum, crimped Temperature RT-300° C. 25- target temperature Heating rate 10° C./min 10° C./min Purge gas N₂ N₂

¹H NMR

¹H NMR data was collected on Bruker 400M NMR Spectrometer using DMSO-d₆.

DVS

DVS was measured via a SMS (Surface Measurement Systems) DVS Intrinsic. Parameters for DVS test are listed in Table 19.

TABLE 19 Parameters for DVS test Parameters Value Temperature 25° C. Sample size 10-20 mg Gas and flow rate N₂, 200 mL/min dm/dt 0.002%/min Min. dm/dt stability duration 10 min Max. equilibrium time 180 min RH range 0% RH-95% RH-0% RH RH step size 10% (0% RH-90% RH-0% RH) 5% (90% RH-95% RH and 95% RH-90% RH)

HPLC

Agilent 1100/1260 HPLC was utilized to analyze purity and solubility, and the detailed method was listed in Table 20.

TABLE 20 HPLC method for purity and solubility test Item Purity Solubility Column Phenomenex Gemini C18 110A, 4.6 × 250 mm, 5.0 μm A: 0.1% FA in H₂O Mobile phase B: 0.1% FA in Acetonitrile Time Time (min) % B (min) % B Gradient table 0.0 5 0.0 5 20.0 100 7.0 100 20.1 5 7.1 5 25.0 5 10.0 5 Run time 25.0 min 10.0 min Post time  0.0 min  0.0 min Flow rate 1.0 mL/min Injection volume 10 μL Detector wavelength UV at 254 nm Column temperature 30° C. Sampler temperature RT Diluent ACN:H₂O (1:1)

IC

IC method for Cl⁻ content measurement was listed in Table 21.

TABLE 21 IC method for Cl⁻ content measurement Item Value Column IonPac AS18 Analytical Column (4 × 250 mm) Mobile phase 25 mM NaOH Injection volume 25 μL Flow rate 1.0 mL/min Injection volume 5 μL Cell temperature 35° C. Column temperature 35° C. Current 80 mA Run time 6.0 min

Example 32 The Preparation of Crystalline Type B of Formula IX

Crystalline Type B of Formula IX was obtained via heating a sample of crystalline Type A to 212° C., cooling to 30° C. under protection of nitrogen and exposing to air conditions. The HPLC purity and stoichiometry (acid:FB) of crystalline Type B were determined to be 97.86 area % and 0.86, respectively. The XRPD pattern is shown in FIG. 40, and TGA/DSC curves are displayed in FIG. 41. The results indicated that Type B was crystalline with a weight loss of 1.2% before 150° C. in TGA and three endothermic peaks at 161.4, 232.2 and 262.3° C. (peak) in DSC. Due to the limited TGA weight loss and neat DSC before 150° C., Type B was speculated to be an anhydrate. To investigate the thermal signal, heating experiment was conducted. As shown in FIG. 42, Type B converted to Type I (Type I is discussed in further detail in Example 38) after being heated to 100° C. or 170° C., cooled to 30° C. under protection of nitrogen, and then exposed to air.

Peak values of the XRPD plot shown in FIG. 40 are provided in Table 22, below.

TABLE 22 XRPD peak values for Crystalline Type B of Formula IX Position [°2θ] Relative Intensity [%] 9.8 2.40 12.0 100.00 13.8 25.37 17.2 12.82 17.7 13.85 18.8 2.52 19.8 38.56 20.7 3.48 21.1 6.60 21.9 2.68 22.6 7.05 23.3 92.78 24.4 21.44 24.8 23.23 26.3 13.18 27.0 3.20 27.7 6.15 28.3 2.26 28.9 14.47 29.9 8.96 30.2 2.79 31.5 1.89 36.1 3.11 37.8 1.64

Example 33 The Preparation of Crystalline Type C of Formula IX

Crystalline Type C of Formula IX was obtained via anti-solvent addition in DMSO/H₂O at RT and its XRPD is shown in FIG. 43. TGA and DSC results in FIG. 44 showed a weight loss of 3.1% up to 150° C. and two endothermic peaks at 91.2 and 173.0° C. Since the Cl⁻ content of Type C sample was 0.17% (the theoretical Cl⁻ content of mono-HCl salt is 10.8%), Type C was confirmed to be a freebase form.

Peak values of the XRPD plot shown in FIG. 43 are provided in Table 23, below.

TABLE 23 XRPD peak values for Crystalline Type C of Formula IX Position [°2θ] Relative Intensity [%] 9.5 10.95 11.7 19.10 12.3 38.53 13.4 19.21 14.6 99.67 15.8 37.90 16.7 79.33 17.2 16.59 17.8 8.43 18.4 6.88 19.5 41.44 20.3 9.55 20.7 21.39 21.4 42.38 21.7 18.03 22.5 37.96 22.9 26.38 24.0 4.72 24.7 20.49 26.1 100.00 26.7 18.16 28.8 8.11 29.6 5.55 30.4 5.96 31.1 5.56 34.4 5.29 35.3 4.44 36.3 2.70 38.1 4.10

Example 34 The Preparation of Crystalline Type D & Type F of Formula IX

Crystalline Type D of Formula IX was obtained via anti-solvent addition in MeOH/IPAc system at RT. Type F of Formula IX was obtained via slurry of Type A in toluene at 50° C. Their XRPD patterns are shown in FIG. 45 (Type D, upper plot; Type F, lower plot).

Peak values of the XRPD plot shown in FIG. 45 (Types D and F) are provided in Table 24 and Table 25, below.

TABLE 24 XRPD peak values for Crystalline Type D of Formula IX Position [°2θ] Relative Intensity [%] 6.8 19.59 10.2 16.65 12.0 71.32 12.3 43.00 13.8 100.00 15.4 26.66 16.6 12.89 18.2 48.41 19.8 13.71 21.7 78.63 22.2 64.23 24.0 26.05 26.0 72.52 26.5 51.29 27.8 21.59 30.8 23.74 34.6 4.15

TABLE 25 XRPD peak values for Crystalline Type F of Formula IX Position [°2θ] Relative Intensity [%] 4.6 6.80 12.4 5.23 12.9 5.29 13.3 22.78 13.8 17.01 14.7 5.10 15.5 22.78 16.3 16.83 18.1 8.86 18.6 18.97 19.0 7.62 20.1 10.89 21.6 5.75 22.3 14.39 22.5 16.27 24.1 100.00 25.2 17.28 25.7 35.11 26.0 34.41 26.4 15.15 27.2 4.40 28.2 6.46 28.9 8.86 29.4 7.40 30.0 15.47 30.6 6.43 31.0 7.51 31.3 5.94 32.0 3.26 34.4 1.81 35.1 1.46 36.3 2.36 37.0 2.00 37.8 3.29

Example 35 The Preparation of Crystalline Type E of Formula IX

Crystalline Type E of Formula IX was obtained via slow evaporation in CHCl₃/EtOH at RT. The HPLC purity and stoichiometry (acid:FB) of crystalline Type E of Formula IX were determined to be 98.60 area % and 0.91, respectively. The XRPD pattern is shown in FIG. 46, and TGA/DSC curves are displayed in FIG. 47. The results indicated that crystalline Type E has a weight loss of 1.5% before 130° C. in TGA and two endothermic peaks at 182.0 and 242.7° C. in DSC (peak). Due to the limited TGA weight loss and neat DSC before 170° C., Type E was speculated to be an anhydrate. To investigate the thermal signal at 182.0° C. (peak) in DSC, heating experiment was conducted. As shown in FIG. 48, Type E converted to hydrate Type I after being heated to 195° C., cooled to 30° C. under protection of nitrogen, and then exposed to air. Based on the thermal data and heating experiment, anhydrate Type E might convert to a new anhydrate form (the DSC endothermic signal ˜180° C. might be a form transition signal) and then the anhydrate form turned into hydrate Type I via interaction with moisture when exposed to ambient condition.

Peak values of the XRPD plot shown in FIG. 46 are provided in Table 26, below.

TABLE 26 XRPD peak values for Crystalline Type E of Formula IX Position [°2θ] Relative Intensity [%] 11.8 29.29 14.0 18.45 15.1 12.00 17.2 2.51 18.2 0.97 19.9 3.73 20.6 2.90 21.4 1.43 24.0 3.14 25.8 100.00 27.8 2.34 29.1 2.06 33.2 1.16

Example 36 The Preparation of Crystalline Type G of Formula IX

Crystalline Type G of Formula IX was obtained via slurry in DMAc/H₂O (v:v, 1:3) at RT and its XRPD is shown in FIG. 49. TGA and DSC results in FIG. 50 showed a weight loss of 3.7% up to 200° C. and one sharp endothermic signal at 231.1° C. (peak). Since the Cl⁻ content of Type G sample was 0.14% (the theoretical Cl⁻ content of mono-HCl salt is 10.8%), Type G was confirmed to be a freebase form.

Peak values of the XRPD plot shown in FIG. 49 are provided in Table 27, below.

TABLE 27 XRPD peak values for Crystalline Type G of Formula IX Position [°2θ] Relative Intensity [%] 6.6 1.33 9.8 3.80 12.2 47.25 13.1 66.55 13.4 46.87 14.6 8.71 15.1 8.08 16.4 3.87 17.1 5.54 17.8 46.61 18.4 14.28 18.7 13.76 19.4 16.96 19.9 8.02 20.3 4.92 21.0 5.62 21.7 32.86 22.8 5.34 23.2 13.61 23.7 8.42 24.3 29.93 24.7 23.96 24.9 25.98 25.4 8.92 26.0 13.52 26.6 100.00 28.3 24.14 29.2 2.64 30.5 8.98 30.9 7.92 33.1 1.62 35.0 1.54 35.7 2.29

Example 37 The Preparation of Crystalline Type H of Formula IX

Crystalline Type H of Formula IX was obtained via slow evaporation in acetone/MeOH system at RT, and its XRPD is shown in FIG. 51. The HPLC purity and stoichiometry (acid:FB) of Type H (810119-11-A4) were determined to be 98.47 area % and 0.91, respectively. TGA and DSC curves (FIG. 52) showed a weight loss of 4.6% before 120° C. and three endothermic peaks at 90.4, 200.5 and 232.3° C. (peak). As shown in the ¹H NMR spectrum (FIG. 53), 0.36 equivalent of MeOH (−3.40 wt %) was detected. Combined with the fact that form change to Type I was observed after Type H was heated to 120° C., cooled to 30° C. under protection of nitrogen (FIG. 54), and exposed to ambient conditions, Form H was speculated to be a MeOH solvate.

Peak values of the XRPD plot shown in FIG. 51 are provided in Table 28, below.

TABLE 28 XRPD peak values for Crystalline Type H of Formula IX Position [°2θ] Relative Intensity [%] 11.8 28.73 12.3 21.19 13.8 14.54 15.7 5.38 16.9 16.03 20.6 4.73 21.7 100.00 23.2 17.57 24.7 12.94 25.7 58.09 26.7 6.83 27.8 9.32 28.1 9.00

Example 38 The Preparation of Crystalline Type I of Formula IX

Crystalline Type I of Formula IX was obtained via heating crystalline Type B of Formula IX to 100° C., cooled to 30° C. under protection of nitrogen, and then exposed to air. Its XRPD is shown in FIG. 55. The HPLC purity and stoichiometry (acid:FB) of crystalline Type I of Formula IX were determined to be 97.94 area % and 0.86, respectively. Since crystalline Type I was obtained via solid-state transition of anhydrate Type B and 3.0% weight loss (equivalent to 0.5 molar water) with endothermic peak at 62.0° C. (peak, FIG. 56) was observed, Type I was speculated to be a hydrate.

Peak values of the XRPD plot shown in FIG. 55 are provided in Table 29, below.

TABLE 29 XRPD peak values for Crystalline Type I of Formula IX Position [°2θ] Relative Intensity [%] 11.4 4.60 12.1 35.57 12.4 89.18 13.6 8.36 13.9 14.40 14.2 10.59 14.8 6.38 16.8 9.64 17.2 41.23 17.8 21.69 18.7 10.82 19.1 9.61 19.9 15.55 20.7 27.63 21.2 8.33 21.7 12.83 22.2 10.01 22.8 18.37 23.3 100.00 24.1 9.53 24.9 41.18 25.4 52.09 25.9 10.04 27.0 26.02 28.3 14.09 29.0 11.22 29.6 8.99 30.0 8.40 30.8 5.50 32.8 6.13 33.3 6.37 33.8 4.22 36.1 1.88 36.9 2.91 37.8 2.89

To further identify crystalline Type I and investigate its dehydration behavior, in-situ XRPD with N₂ flow was performed to observe the dehydrated form of Type I, and KF test was conducted to confirm whether the TGA weight loss was caused by water content or not. As shown in FIG. 57, form change to anhydrate crystalline Type B was observed for crystalline Type I with N₂ purging for about 1.5 hr. (30° C./16% RH). As shown in FIG. 58 a weight loss of 2.6% up to 120° C. was observed in crystalline Type I. Based on the KF result, around 3.48% water content was observed in the crystalline Type I sample. Combined with the form change to anhydrate Type B under N₂ flow, Type I was identified to be a hydrate.

Example 39 The Preparation of Crystalline Type J of Formula IX

Crystalline Type J of Formula IX was obtained by slow evaporation followed by vacuum drying at 50° C. in MEK/DMAc system, and its XRPD is shown in FIG. 59. The HPLC purity and stoichiometry (acid:FB) of crystalline Type J of Formula IX was determined to be 91.69 area % and 0.90, respectively. TGA and DSC results in FIG. 60 showed a weight loss of 21.5% up to 120° C. and three endothermic peaks at 120.8, 197.8 and 221.5° C. (peak). As shown in the ¹H NMR spectrum (FIG. 61), 4.9 equivalent of DMAc (56.51 wt %) was detected. Combined with the fact that form change to a mixture of crystalline Type I (highlighted) and crystalline Type A was observed after crystalline Type J was heated to 130° C., cooled to 30° C. under protection of nitrogen, and exposed to ambient conditions (FIG. 62), Type J was speculated to be a DMAc solvate.

Peak values of the XRPD plot shown in FIG. 59 are provided in Table 30, below.

TABLE 30 XRPD peak values for Crystalline Type J of Formula IX Position [°2θ] Relative Intensity [%] 4.6 18.19 11.8 8.94 12.8 9.10 13.8 87.83 14.6 29.55 18.4 26.65 20.5 18.63 21.1 8.10 21.9 8.04 22.8 100.00 23.7 18.41 26.2 33.09 27.7 35.16 28.8 29.02 30.4 12.86 30.8 8.86 31.3 6.46 32.6 8.32 36.0 3.83

Example 40 The Preparation of Crystalline Type K of Formula IX

Free base material as prepared in Example 11 was characterized by XRPD (FIG. 63), TGA (FIG. 64), and DSC (FIG. 64). This material was referred to as crystalline Type K of Formula IX. A weight loss of 6.1% up to 150° C. was observed in TGA, and DSC result showed endothermic peaks at 159.3, 176.2 and 278.4° C. (peak). The HPLC purity of crystalline Type K of Formula IX was determined to be 99.12 area %.

Peak values of the XRPD plot shown in FIG. 63 are provided in Table 31, below.

TABLE 31 XRPD peak values for Crystalline Type K of Formula IX Position [°2θ] Relative Intensity [%] 7.1 21.72 7.5 20.44 9.8 11.27 13.9 100.00 15.9 6.59 19.3 7.18 20.9 2.30 21.9 7.51 22.7 5.79 23.0 3.40 24.0 2.73 24.9 3.09 25.5 1.35 27.3 2.70 28.1 1.86 29.1 1.70 35.0 1.79

Although the foregoing has been described in some detail by way of illustration and example for purposes of clarity and understanding, one of skill in the art will appreciate that certain changes and modifications can be practiced within the scope of the appended claims. In addition, each reference provided herein is incorporated by reference in its entirety to the same extent as if each reference was individually incorporated by reference. 

What is claimed is:
 1. A pharmaceutically acceptable salt represented by Formula I

wherein X is a pharmaceutically acceptable anion of a protic acid.
 2. The pharmaceutically acceptable salt of claim 1, wherein the pharmaceutically acceptable anion of a protic acid is selected from the group consisting of chloride, bromide, sulfonate, tosylate, mesylate, nitrate and acetate, and combinations thereof.
 3. The pharmaceutically acceptable salt of claim 2, wherein the pharmaceutically acceptable anion of a protic acid is mesylate.
 4. The pharmaceutically acceptable salt of claim 2, wherein the pharmaceutically acceptable anion of a protic acid is tosylate.
 5. The pharmaceutically acceptable salt of claim 2, wherein the pharmaceutically acceptable anion of a protic acid is chloride.
 6. A pharmaceutically acceptable salt represented by Formula Ib

wherein Y is a multiprotic acid.
 7. The pharmaceutically acceptable salt of claim 2, wherein Y is selected from the group consisting of ethane-1,2,-disulfonic acid, sulfuric acid, citric acid, maleic acid, malic acid, tartaric acid, and oxalic acid.
 8. A method for preparing a compound of Formula IX

comprising (a) contacting a compound of Formula II

with a compound of Formula III

a first base, and a first organic solvent to provide a compound of Formula IV

wherein each R¹ is independently a halogen or a sulfonate ester; (b) contacting a compound of Formula IV with hydrazine and a second organic solvent to provide a compound of Formula V

(c) contacting a compound of Formula V with a sulfonating agent or halogenating agent provide a compound of Formula VI

wherein R² is a halogen or a sulfonate ester; (d) contacting a compound of Formula VI with a compound of Formula VII

a second base, and an third organic solvent to provide a compound of Formula VIII

wherein R³ is selected from the group consisting of an C₁₋₁₂ alkyl, C₂₋₁₂ alkenyl, C₁₋₁₂ alkynyl, C₃₋₈ cycloalkyl, C₃₋₁₂ heterocycloalkyl, aryl, and heteroaryl, each of which is optionally substituted. (e) contacting a compound of Formula VIII with a third base to provide a compound of Formula IX.
 9. The method of claim 8, wherein each R¹ is independently selected from the group consisting of chloride, bromide, tosylate, and mesylate.
 10. The method of claim 9, wherein each R¹ is bromide.
 11. The method of any one of claims 8 to 10, wherein the first base is an alkali metal carbonate, an alkali metal bicarbonate or a combination thereof.
 12. The method of claim 9, wherein the alkali metal carbonate is selected from the group consisting of Li₂CO₃, Na₂CO₃, and K₂CO₃, or a combination thereof.
 13. The method of claim 12, wherein the alkali metal carbonate is K₂CO₃.
 14. The method of claim 11, wherein the alkali metal bicarbonate is selected from the group consisting of LiHCO₃, NaHCO₃, and KHCO₃,or a combination thereof
 15. The method of any one of claims 8 to 14, wherein the first organic solvent is a polar aprotic organic solvent.
 16. The method of claim 15, wherein the polar organic solvent is selected from the group consisting of acetone, ethyl acetate, dichloromethane, tetrahydrofuran, dimethylformamide and acetonitrile.
 17. The method of claim 16, wherein the polar organic solvent is acetone.
 18. The method of any one of claims 8 to 17, wherein the second organic solvent is a polar protic organic solvent.
 19. The method of claim 18, wherein the polar protic organic solvent is C₁₋₁₀—OH.
 20. The method of claim 19, wherein the polar protic organic solvent is ethanol.
 21. The method of any one of claims 8 to 20, wherein the sulfonating agent is mesyl chloride or tosyl chloride.
 22. The method of any one of claims 8 to 20, wherein the halogenating agent is selected from the group consisting of PBr₃, PCl₃, PCl₅, SOBr₂, PBr₅, and SOCl₂.
 23. The method of claim 22, wherein the halogenating agent is PBr₃.
 24. The method of any one of claims 8 to 23, wherein R² is selected from the group consisting of chloride, bromide, tosylate, and mesylate.
 25. The method of claim 24, wherein R² is Br.
 26. The method of any one of claims 8 to 25, wherein the second base is an alkali metal carbonate, an alkali metal bicarbonate or a combination thereof
 27. The method of claim 25, wherein the alkali metal carbonate is selected from the group consisting of Li₂CO₃, Na₂CO₃, and K₂CO₃, or a combination thereof.
 28. The method of claim 27, wherein the alkali metal carbonate is K₂CO₃.
 29. The method of claim 25, wherein the alkali metal bicarbonate is selected from the group consisting of LiHCO₃, NaHCO₃, and KHCO₃,or a combination thereof
 30. The method of any one of claims 8 to 29, wherein the third organic solvent is a polar aprotic organic solvent.
 31. The method of claim 30, wherein polar organic solvent is selected from the group consisting of acetone, ethyl acetate, dichloromethane, tetrahydrofuran, dimethylformamide, dimethylsulfoxide and acetonitrile.
 32. The method of claim 31, wherein polar organic solvent is dimethylformamide.
 33. The method of claim 31, wherein polar organic solvent is dimethylsulfoxide.
 34. The method of any one of claims 8 to 32, wherein R³ is C₁₋₈ alkyl.
 35. The method of claim 34, wherein R³ is methyl.
 36. The method of any one of claims 8 to 35, wherein the third base is a metal hydroxide.
 37. The method of claim 36, wherein the metal hydroxide is an alkali metal hydroxide.
 38. The method of claim 37, wherein the alkali metal hydroxide is a selected from the group consisting of LiOH, NaOH, KOH, RbOH, and CsOH.
 39. The method of claim 38, where in the alkali metal hydroxide is LiOH.
 40. The method of claim 38, where in the alkali metal hydroxide is NaOH.
 41. Crystalline Type A of Formula IX characterized by an X-ray powder diffraction pattern comprising peaks at 12.0, 21.8, 25.9, 26.7, and 27.9 degrees 2θ (±0.2 degrees 2θ).
 42. Crystalline Type A of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG.
 31. 43. Crystalline Type A of Formula IX of claim 41 or 42, which is substantially free of other Formula IX crystalline or amorphous forms.
 44. Crystalline Type A of Formula IX according to any one of claims 41 to 42, further characterized by a weight loss ranging from about 0.7% to about 1.9% upon heating to around 150° C., as measured by thermal gravimetric analysis.
 45. Crystalline Type A of Formula IX according to claim 44, wherein the weight loss is about 1.3%.
 46. Crystalline Type A of Formula IX characterized by water uptake of about 1.6% at 25° C./80% relative humidity (RH) after undergoing a dynamic vapor sorption cycle which includes pre-equilibration at 0% RH.
 47. Crystalline Type A of Formula IX characterized by gains of less than 2.5% weight after undergoing a dynamic vapor sorption cycle from about 0% relative humidity (RH) to about 90% RH.
 48. Crystalline Type A of Formula IX having a dynamic vapor sorption profile substantially as shown in FIG.
 37. 49. Crystalline Type A of Formula IX according to any one of claims 41 to 45, further characterized by a differential scanning calorimetry thermogram comprising endothermic peaks at around 211-214 and 237-239° C.
 50. Crystalline Type A of Formula IX according to claim 49, wherein, said differential scanning calorimetry thermogram comprises endothermic peaks around 11.7, 212.6, and 237.3° C.
 51. Crystalline Type B of Formula IX characterized by an X-ray powder diffraction pattern comprising peaks at 12.1, 13.9, 19.8, 23.3, and 24.4 degrees 2θ (±0.2 degrees 2θ).
 52. Crystalline Type B of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG.
 40. 53. Crystalline Type B of Formula IX of claim 51 or 52, which is substantially free of other Formula IX crystalline or amorphous forms.
 54. Crystalline Type B of Formula IX according to claim 51 or 52, further characterized by a weight loss ranging from about 0.6% to about 2.0% upon heating to around 150° C., as measured by thermal gravimetric analysis.
 55. Crystalline Type B of Formula IX according to claim 51 or 52, further characterized by a weight loss of about 1.2% upon heating to around 150° C., as measured by thermal gravimetric analysis.
 56. Crystalline Type B of Formula IX according to any one of claims 51 to 54, further characterized by a differential scanning calorimetry thermogram comprising endothermic peaks at around 161.4, 232.2 and 262.3° C.
 57. Crystalline Type E of Formula IX characterized by an X-ray powder diffraction pattern comprising peaks at 11.9, 14.0, 15.1, and 25.8 degrees 2θ (±0.2 degrees 2θ).
 58. Crystalline Type E of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG.
 46. 59. Crystalline Type E of Formula IX of claim 57 or 58, which is substantially free. of nther Formula IX crystalline or amorphous forms.
 60. Crystalline Type E of Formula IX according to claim 57 or 58, further characterized by a weight loss ranging from about 0.5% to about 2.5% upon heating to around 150° C., as measured by thermal gravimetric analysis.
 61. Crystalline Type E of Formula IX according to claim 57 or 58, further characterized by a weight loss of about 1.5% upon heating to around 150° C., as measured by thermal gravimetric analysis.
 62. Crystalline Type E of Formula IX according to any one of claims 57 to 60, further characterized by a differential scanning calorimetry thermogram comprising endothermic peaks at around 182.0 and 242.7° C.
 63. Crystalline Type I of Formula IX characterized by an X-ray powder diffraction pattern comprising peaks at 12.5, 17.3, 23.4, 25.0, and 25.4 degrees 2θ (±0.2 degrees 2θ).
 64. Crystalline Type I of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG.
 55. 65. Crystalline Type I of Formula IX of claim 63 or 64, which is substantially free of other Formula IX crystalline or amorphous forms.
 66. Crystalline Type I of Formula IX according to claim 63 or 64, further characterized by a weight loss ranging from about 2.5% to 3.5% upon heating to around 120° C., as measured by thermal gravimetric analysis.
 67. Crystalline Type I of Formula IX according to claim 63 or 64, further characterized by a weight loss of about 3.0% upon heating to around 120° C., as measured by thermal gravimetric analysis.
 68. Crystalline Type I of Formula IX according to any one of claims 63 to 66, further characterized by a differential scanning calorimetry thermogram comprising endothermic peaks at around 62.0 and 158.4, 215.7° C.
 69. Crystalline Type H of Formula IX characterized by an X-ray powder diffraction pattern comprising peaks at 11.9, 12.3, 21.7, 23.3, and 25.8 degrees 2θ (±0.2 degrees 2θ).
 70. Crystalline Type H of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG.
 51. 71. Crystalline Type H of Formula IX of claim 69 or 70, which is substantially free of other Formula IX crystalline or amorphous forms.
 72. Crystalline Type H of Formula IX according to claim 69 or 70, further characterized by a weight loss ranging from about 3.5% to about 5.5% upon heating to around 150° C., as measured by thermal gravimetric analysis.
 73. Crystalline Type H of Formula IX according to claim 69 or 70, further characterized by a weight loss of about 4.6% upon heating to around 150° C., as measured by thermal gravimetric analysis.
 74. Crystalline Type H of Formula IX according to any one of claims 69 to 73, further characterized by a differential scanning calorimetry thermogram comprising endothermic peaks at around 90.4 and 200.5, 232.3° C.
 75. Crystalline Type J of Formula IX characterized by an X-ray powder diffraction pattern comprising peaks at 13.8, 14.7, 22.9, 26.2, and 27.7degrees 2θ (±0.2 degrees 2θ).
 76. Crystalline Type J of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG.
 59. 77. Crystalline Type J of Formula IX of claim 75 or 76, which is substantially free of other Formula IX crystalline or amorphous forms.
 78. Crystalline Type J of Formula IX according to claim 75 or 76, further characterized by a weight loss ranging from about 17.5% to about 24% upon heating to around 120° C., as measured by thermal gravimetric analysis.
 79. Crystalline Type J of Formula IX according to claim 75 or 76, further characterized by a weight loss of about 21.5% upon heating to around 120° C., as measured by thermal gravimetric analysis.
 80. Crystalline Type J of Formula IX according to any one of claims 75 to 79, further characterized by a differential scanning calorimetry thermogram comprising endothermic peaks at around 120.8 and 197.8, 221.5° C.
 81. Crystalline Type K of Formula IX characterized by an X-ray powder diffraction pattern comprising peaks at 7.2, 7.6, 9.9, 14.0, and 19.3 degrees 2θ (±0.2 degrees 2θ).
 82. Crystalline Type K of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG.
 59. 83. Crystalline Type K of Formula IX of claim 81 or 82, which is substantially free of other Formula IX crystalline or amorphous forms.
 84. Crystalline Type K of Formula IX according to claim 81 or 82, further characterized by a weight loss ranging from about 5.0% to about 7.0% upon heating to around 1 70° C., ac mpasurpri by thermal gravimetric analysis.
 85. Crystalline Type K of Formula IX according to claim 81 or 82, further characterized by a weight loss ranging from about 6.1% upon heating to around 120° C., as measured by thermal gravimetric analysis.
 86. Crystalline Type K of Formula IX according to any one of claims 81 to 85, further characterized by a differential scanning calorimetry thermogram comprising endothermic peaks at around 159.3 and 176.2, 278.4° C.
 87. Crystalline Type C of Formula IX characterized by an X ray powder diffraction pattern comprising peaks at 14.6, 16.8, 19.5, 20.7, and 22.5 degrees 2θ (±0.2 degrees 2θ).
 88. Crystalline Type C of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG.
 43. 89. Crystalline Type C of Formula IX of claim 87 or 88, which is substantially free of other Formula IX crystalline or amorphous forms.
 90. Crystalline Type C of Formula IX according to claim 87 or 88, further characterized by a weight loss ranging from about 2.0% to about 4.0% upon heating to around 150° C., as measured by thermal gravimetric analysis.
 91. Crystalline Type C of Formula IX according to claim 87 or 88, further characterized by a weight loss of about 3.1% upon heating to around 150° C., as measured by thermal gravimetric analysis.
 92. Crystalline Type C of Formula IX according to any one of claims 87 to 91, further characterized by a differential scanning calorimetry thermogram comprising endothermic peaks at around 91.2 and 173.0° C.
 93. Crystalline Type G of Formula IX characterized by an X-ray powder diffraction pattern comprising peaks at 12.3, 13.2, 13.4, 17.8, and 26.6 degrees 2θ (±0.2 degrees 2θ).
 94. Crystalline Type G of Formula IX characterized by an X-ray powder diffraction pattern substantially in accordance with FIG.
 43. 95. Crystalline Type G of Formula IX of claim 93 or 94, which is substantially free of other Formula IX crystalline or amorphous forms.
 96. Crystalline Type G of Formula IX according to claim 93 or 94, further characterized by a weight loss ranging from about 1.7% to about 2.7% upon heating to around 200° C., as measured by thermal gravimetric analysis.
 97. Crystalline Type G of Formula IX according to claim 93 or 94, further characterized by a weight loss of about 3.7% upon heating to around 200° C., as measured by thermal gravimetric analysis.
 98. Crystalline Type G of Formula IX according to any one of claims 93 to 97, further characterized by a differential scanning calorimetry thermogram comprising endothermic peaks at around 231.1° C. 